会影响线粒体功能,导致乌初级肝细胞的亡和自
Shuqin Lin1, Yunjuan Xiao1, Jing Lin1
1Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou 571158, China.
Animals : an open access journal from MDPI
|August 29, 2024
概括
六价 (Cr(VI)) 暴露会诱导氧化应激,线粒体功能障碍,亡,以及里夫斯的自.
科学领域:
- 环境毒理学环境毒理学
- 细胞生物学 细胞生物学
- 类学 类学 类学 类学
背景情况:
- 六值 (Cr) 是一种广泛存在的工业污染物,已知有毒性.
- (VI) 对海的毒理影响在很大程度上仍未得到研究.
- 里夫斯海 (Mauremys reevesii) 是研究海健康的相关模型.
研究的目的:
- 为了研究Cr(VI) 对从里夫斯海中分离出来的初级肝细胞的毒性作用.
- 阐明在乌肝细胞中的Cr (VI) 毒性背后的分子机制.
主要方法:
- 里夫斯海的初级肝细胞被暴露在25微米和50微米的Cr (VI) 中24小时.
- 进行了测试,以测量抗氧化酶活性,活性氧物种 (ROS) 水平和腺三酸盐 (ATP) 水平.
- 对与线粒体动力学,亡和自相关的基因进行了基因表达分析.
主要成果:
- (Cr) 的暴露增加了抗氧化酶活性 (catalase,超氧化物失调酶) 和ROS水平.
- 线粒体功能障碍是由ATP水平的降低和线粒体动力学基因 (Mfn2,SIRT1,SIRT3,p53) 的改变表达的迹象.
- ) 促进了亡 (增加了Bax,Caspase3;减少了Bcl2) 和调节了自途径 (改变了LC3,ULK1,p62,mTOR,Beclin1表达).
结论:
- 在海肝细胞中,Cr(VI) 诱导氧化应激和线粒体功能障碍.
- 这些细胞变化导致亡和自的增加.
- 这些发现强调了Cr (VI) 污染对野生海种群的健康风险,以及需要采取保护措施.
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