猫脂肪组织衍生的介质干细胞/干细胞在体外传播过程中的形态,增殖和细胞遗传变化
Agustina Algorta1, Rody Artigas2, Analía Rial3
1Unidad de Inmunología e Inmunoterapia, Departamento de Patobiología, Facultad de Veterinaria, Universidad de la República (UdelaR), Montevideo 13000, Uruguay.
Animals : an open access journal from MDPI
|August 29, 2024
概括
来自猫脂肪组织的介质细胞/干细胞 (MSCs) 在体外通过4后显示出减少的增殖和增加的异常. 这影响了它们适用于需要大量细胞扩张的猫类干细胞治疗的适用性.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 再生医学是一种再生医学.
- 细胞生物学 细胞生物学
背景情况:
- 猫类干细胞疗法需要大量的介质干细胞/干细胞 (MSC).
- 在体外扩展MSC可以改变其特征和遗传稳定性.
- 猫脂肪组织衍生MSCs (cAT-MSCs) 是猫疗法的潜在来源.
研究的目的:
- 为了评估cAT-MSCs的体外特征.
- 在延长培养期间评估cAT-MSCs的细胞遗传稳定性.
- 为了确定体外繁殖对cAT-MSC质量对潜在治疗用途的影响.
主要方法:
- 评估了cAT-MSCs在2,4和6段的形态特征,克隆性潜力和繁殖能力.
- 评估的多功效和免疫类型 (CD45-, CD90+, CD44+).
- 进行了细胞遗传学分析,包括转相分析,直到通道6.
主要成果:
- cAT-MSCs表现出增殖能力下降和经过后翻倍时间增加4.
- 在通道4观察到核异常 (例如,集群形成,肌,肌溶解) 和降低的线粒体指数.
- 检测到结构性染色体不稳定性,但并没有随着传递而增加.
结论:
- 通过后,cAT-MSCs的繁殖能力显著下降4.通过后.
- 在体外培养诱导了cAT-MSCs的形态变化和结构不稳定的迹象.
- 这些发现表明,对于猫类干细胞应用中cAT-MSCs的广泛体外扩展存在局限性.
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