重温折叠变化计算:偏好中位数或几何平均值而不是以算术平均值为基础的方法
Jörn Lötsch1,2,3, Dario Kringel1, Alfred Ultsch4
1Institute of Clinical Pharmacology, Goethe University, Theodor Stern Kai 7, 60590 Frankfurt am Main, Germany.
Biomedicines
|August 29, 2024
概括
计算omics数据中的折叠变化的算术平均值方法是不可靠的. 强大的方法,如中位数或几何平均值,可以提高生物医学研究的准确性和可重复性.
科学领域:
- 生物统计学 生物统计学
- 生物信息学是一种生物信息学.
- 基因组学就是基因组学.
- 蛋白质组学是指蛋白质组学.
- 代谢学 代谢学 代谢学
背景情况:
- 折叠变化是分析奥米克数据的关键指标.
- 不一致的折叠变化的计算和报告引入了差异.
- 这项研究解决了对标准化折叠变换方法的需求.
研究的目的:
- 评估各种折叠变化计算方法.
- 为了确定一个首选的,强大的方法对OMIC数据分析.
- 提高生物医学研究成果的可复制性.
主要方法:
- 生成的人工数据集具有不同的分布 (例如,正常,日志-正常).
- 与模拟数据中的已知值进行折叠变化计算的比较.
- 分析了多omics数据集,以评估现实世界的适用性.
主要成果:
- 基于算术平均值的折叠变化计算经常不准确.
- 不准确性表现在不同的子组分布或标准偏差.
- 其他方法 (中位数,几何平均值) 显示出更大的稳定性.
结论:
- 算术平均值是计算折叠变化的劣质方法.
- 中位数,几何平均值或配对折叠变换方法提供了更好的可靠性.
- 标准化,强大的折叠变化计算和透明的报告对于准确的解释和可重复性至关重要.
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