血清白丰富的α-2-糖蛋白1在儿科急性尾炎的诊断性能:一项前性验证研究
Javier Arredondo Montero1, Raquel Ros Briones2, Amaya Fernández-Celis3
1Pediatric Surgery Department, Complejo Asistencial Universitario de León, 24008 León, Spain.
血清白蛋白丰富的α-2-糖蛋白1 (LRG-1) 显示出作为儿科急性尾炎 (PAA) 的生物标志物具有前途. 升高的LRG-1水平可以帮助诊断PAA,并区分复杂和不复杂的病例.
科学领域:
- 生物化学 生物化学
- 儿科医学 儿科医学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 富含白的α-2-糖蛋白1 (LRG-1) 是一种具有作为诊断生物标记物的潜力的人类蛋白质.
- 儿童急性尾炎 (PAA) 诊断可能具有挑战性,需要改进的诊断工具.
研究的目的:
- 在儿科患者中验证血清LRG-1的诊断性能.
- 在儿科队列中建立规范性血清LRG-1范围.
- 评估LRG-1区分PAA与其他腹部疼痛原因的能力,并区分复杂和无复杂的PAA.
主要方法:
- 预期BIDIAP队列的子组分析包括200名儿科患者.
- 患者被分为健康对照组,非手术性腹痛组和PAA组.
- 血清LRG-1度使用酶相关免疫吸收试验 (ELISA) 进行测量.
- 统计分析包括曼-惠特尼U,克鲁斯卡尔-瓦利斯,费舍尔的精确测试和ROC曲线分析 (AUC).
主要成果:
- 与健康对照组 (23,145 ng/mL) 和非手术腹痛组 (27,655 ng/mL) 相比,PAA患者 (40,409 ng/mL) 的血清LRG-1水平显著更高 (p < 0.0001).
- 复杂的PAA (51,857 ng/mL) 与非复杂的PAA (38,686 ng/mL) (p = 0.02) 中的LRG-1水平也较高.
- 区分PAA与非手术腹痛的AUC为0.75,复杂PAA与非复杂PAA的AUC为0.66.
结论:
- 血清LRG-1是诊断儿童PAA的潜在有价值的生物标志物.
- 这项研究为儿科患者提供了规范性血清LRG-1值.
- 建议进行进一步的评估血清,尿液和唾液中LRG-1的多中心研究,以评估临床适用性.
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