干燥加工子油对子分离蛋白的结构和物理化学性能的影响
Xiaoyan Liu1, Duwei Yang2, Wantong Liu3
1Hainan Engineering Center of Coconut Further Processing, Coconut Research Institute of Chinese Academy of Tropical Agricultural Sciences, Wenchang 571339, China.
干燥加工的子油影响子分离蛋白质的特性. 这种方法增强了热稳定性,并改善了保持水,保持油和泡的稳定性,为粉蛋白质利用提供了价值.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 生物化学 生物化学
背景情况:
- 子分离蛋白 (CIP) 是子油提取的有价值的副产品.
- 了解处理对CIP功能的影响对于其更广泛的应用至关重要.
- 干燥加工是食品生产中常见的一种方法,但它对CIP的具体影响需要详细调查.
研究的目的:
- 研究子油干加工对子分离蛋白的物理化学和功能性质的影响.
- 确定干燥加工如何影响氨基酸组成,蛋白质结构和热特性.
- 评估保持水,保持油,发泡和乳化能力的变化.
主要方法:
- 氨基酸组成的分析.
- 用于分子量和降解评估的SDS-PAGE.
- 二次结构和表面疏水性评价的光谱方法.
- 测量可溶性,硫含量和热特性 (DSC).
- 对持水性,持油性,发泡性和乳化性能的评估.
主要成果:
- 干燥加工改变了氨基酸组成,导致更均的颗粒大小和提高了CIP的热稳定性.
- SDS-PAGE显示球状蛋白质的轻微降解 (~34 kDa).
- 观察到降低了表面疏水性,硫基,溶解性和自由氨基酸含量.
- 持水能力,持油能力和泡稳定性呈现出不同程度的改善.
结论:
- 子油的干燥加工显著改变了子分离蛋白质的特性.
- 观察到的功能性质的改善表明,可以更好地利用铜粉蛋白.
- 这项研究强调了加工方法在最大限度地提高CIP等副产品的附加值方面的重要性.
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