酵解Corynebacterium glutamicum对大豆蛋白分离物的酶化水解酸的挥发性风味的影响
Lingling Shangguan1, Zixiong Liu1, Linglong Xu1
1Key Laboratory of Fermentation Engineering (Ministry of Education), Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Hubei Key Laboratory of Industrial Microbiology, School of Life Sciences and Health, Hubei University of Technology, Wuhan 430068, China.
使用Corynebacterium glutamicum调味豆蛋白分离物 (SPI) 的微生物发酵增强了味道. 这种新的方法产生了芳香和烤的音符,在调味品中创造了一个独特的肉香气.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 传统的大豆蛋白分离物 (SPI) 的酶性水解可以导致调味品的不良风味.
- 开发新的方法来改善SPI调味品的风味对于食品工业至关重要.
研究的目的:
- 通过微生物发酵开发一种新的SPI调味品,以提高风味质量.
- 调查Corynebacterium glutamicum发酵对SPI酶化水解中的调味剂化合物的影响.
主要方法:
- 使用Corynebacterium glutamicum的SPI酶化水解物的微生物发酵.
- 感官评估和电子鼻子分析.
- 气色谱-质谱 (GC-MS) 用于挥发性化合物的识别.
- 相对气味活性值 (ROAV) 分析以确定关键香料化合物.
主要成果:
- 发酵的SPI调味品呈现出独特的芳香和烤的风味.
- 在传感器响应 (S18,S24,S25) 中观察到显著差异.
- GC-MS确定了91种挥发性化合物,其中发酵的SPI含有更多的酒精,子,芳和异环环.
- 关键的调味化合物 (n-pentylpyrazine,2,6-dimethylpyrazine,tetramethylpyrazine) 被确定为ROAV≥1,有助于烤肉和肉味的香味.
结论:
- 科里尼细菌 (Corynebacterium glutamicum) 发酵显著提高了SPI调味品的风味质量.
- 这个过程为开发和生产增强调味产品提供了一种新的方法.
- 已识别的关键化合物提供了关于发酵的SPI调味品特有的风味特征的见解.
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