保加利亚的水:各种临床样本,临床表现和分子检测的重要性
Stefka Krumova1, Radostina Stefanova1, Petia Genova-Kalou2
1National Reference Laboratory "Measles, Mumps, Rubella", Department of Virology, National Center of Infectious and Parasitic Diseases, 1233 Sofia, Bulgaria.
Journal of clinical medicine
|August 29, 2024
概括
在保加利亚患者中检测到麻疹病毒 (MPXV) DNA.
科学领域:
- 病毒学 病毒学
- 传染性疾病 传染性疾病
- 公共卫生 公共卫生
背景情况:
- 麻疹 (mpox) 是由麻疹病毒 (MPXV) 引起的,它是一种 ортопокс病毒 (OPXV),是一种重要的动物传播疾病.
- 自2022年以来,在全球范围内观察到MPXV的非典型人与人传播,因此需要在非流行地区进行研究.
研究的目的:
- 确定从保加利亚患者的临床样本中检测MPXV和OPXVDNA的频率.
- 评估在急性和康复期mopox感染阶段的推和替代样本类型的检测率.
主要方法:
- 实时PCR被用于分析42名怀疑有mpox的患者的181个临床样本.
- 样本包括囊泡含量,鼻/鼻分泌物,尿液,便,射精和唾液.
主要成果:
- 在12.71% (23/181) 的样本中检测到MPXV DNA,在11.05% (20/181) 的样本中检测到OPXV DNA.
- 在症状出现的前三天内,MPXV/OPXV DNA 经常在囊泡含量和呼吸道分泌物中被发现.
- 在其他样本 (尿液,便,射精,唾液) 中也检测到病毒DNA,并在康复期间在尿液和膀含量中持续超过两周.
结论:
- 比以前想象的更广泛的临床材料中可以检测到MPXV.
- 病毒在某些替代样本类型中表现出长时间的泄露,影响了诊断和公共卫生策略.
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