相关实验视频

Updated: Jun 25, 2026

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

表面等离子体共振免疫传感器用于直接检测针对SARS-CoV-2核囊蛋白抗体

Viktorija Lisyte¹, Asta Kausaite-Minkstimiene¹, Benediktas Brasiunas¹

¹NanoTechnas-Center of Nanotechnology and Materials Science, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania.

International journal of molecular sciences

|August 29, 2024

概括

这项研究开发了一种直接的,无标签的免疫传感器,用于检测针对SARS-CoV-2核体蛋白 (anti-SCoV2-rN) 的抗体. 这种新方法提供了敏感和特定的COVID-19诊断.

科学领域:

生物医学工程生物医学工程
免疫技术是一种免疫技术.
诊断检测试验诊断检测试验

背景情况:

SARS-CoV-2核体蛋白具有高度免疫性,使其抗体对COVID-19诊断至关重要.
对抗SARS-CoV-2核蛋白 (anti-SCoV2-rN) 抗体的准确检测对于患者的诊断至关重要.

研究的目的:

开发一种直接的,无标签的,敏感的免疫传感器,用于检测抗SCoV2-rN抗体.
为了优化复合SARS-CoV-2核蛋白 (SCoV2-rN) 的固定,以提高免疫传感器的性能.

主要方法:

用碳胺化学和自组装单层将SCoV2-rN固定在SPR传感器芯片上.
描述SCoV2-rN表面质量度和抗体结合亲和力 (K_D).
对抗SCoV2-rN.的免疫传感器的线性范围,检测极限 (LOD) 和量化极限 (LOQ) 的评估.

主要成果:

最佳的SCoV2-rN固定实现了3.61±0.52 ng/mm2的表面质量度.
免疫传感器对单克隆抗SCoV2-rN抗体具有很好的亲和力,K_D值为6.49 × 10-8 ± 5.3 × 10-9 M.
建立了0.550nm的线性检测范围,LOD和LOQ值分别为0.057nm和0.19nm. 该试验显示出良好的可重复性和特异性.

结论:

开发的无标签免疫传感器提供了一种敏感和特定的方法来检测抗SCoV2-rN抗体.
这项技术适用于多个抗体检测,并有可能改善COVID-19诊断.

关键词:

在SARS-CoV-2病毒中,核囊蛋白是一种蛋白质.抗体是一种抗体.免疫传感器是一种免疫传感器.表面的等离子体共振.

更多相关视频

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Published on: August 12, 2021

相关概念视频

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.