在大鼠模型中皮肤暴露于子[a]烯的影响:对表皮细胞功能和排水淋巴结细胞反应的洞察
Anastasija Malešević1, Dina Tucović1, Jelena Kulaš1
1Immunotoxicology Group, Department of Ecology, Institute for Biological Research "Siniša Stanković", National Institute of Republic of Serbia, University of Belgrade, 142 Bulevar Despota Stefana, 11000 Belgrade, Serbia.
International journal of molecular sciences
|August 29, 2024
概括
皮肤暴露在空气污染物[a]烯 (BaP) 中会破坏皮肤结构和免疫细胞功能. 这项研究揭示了BaP会引起氧化应激,并改变细胞因子的产生,导致局部免疫抑制和皮肤免疫反应的减少.
科学领域:
- 环境毒理学环境毒理学
- 皮肤病学 皮肤病学
- 免疫学 免疫学 免疫学
背景情况:
- 甲 (BaP) 是一种众所周知的致癌物质,向皮肤.
- 皮肤BaP暴露的免疫毒性影响尚未完全理解.
- 环境和职业接触BAP是一个重要的健康问题.
研究的目的:
- 研究皮肤上BaP应用对皮肤免疫力的免疫调节作用.
- 分析BaP对表皮和淋巴结免疫细胞活动的影响.
- 评估BaP对皮肤对化学敏感性反应的影响.
主要方法:
- 在10天的皮肤上应用BaP的相关剂量.
- 皮肤扩展物,表皮细胞和排水淋巴结 (DLN) 细胞的活体分析.
- 在丁二 (DNCB) 敏感化后对皮肤免疫反应的评估.
主要成果:
- BaP破坏了表皮结构,增加了皮肤免疫细胞的透.
- 在表皮细胞中,BaP诱导了氧化应激,改变了谷氨和酶表达 (AHR,Cyp1A1).
- 在表皮细胞中,BaP调节了细胞因子的产生 (TNF,IL-1β降低;IL-10增加),在DLN细胞中改变了细胞因子的配置.
- 暴露于BaP导致局部免疫抑制,在DNCB敏感化后降低DLN细胞活性.
结论:
- 皮肤BaP暴露显著影响皮肤免疫恒常性.
- BaP诱导氧化应激并改变皮肤中的炎症反应.
- 暴露于BaP可以导致局部免疫抑制,可能会损害皮肤对其他刺激的反应能力.
- 了解这些免疫毒性影响对于评估与BaP相关的健康风险至关重要.
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