在小鼠卵细胞中不对称的分裂时组装Promyelocytic白血病核体的行为
Osamu Udagawa1, Ayaka Kato-Udagawa1, Seishiro Hirano1
1Environmental Risk and Health Research Division, National Institute for Environmental Studies, Tsukuba 305-8506, Japan.
International journal of molecular sciences
|August 29, 2024
概括
在小鼠卵细胞中诱导的原细胞白血病核体 (PML-NBs) 在压力下在细胞质中持续存在. 胚胎清除了这些身体,表明细胞质清除是卵细胞质量和成功受精的关键.
科学领域:
- 细胞生物学 细胞生物学
- 生殖生物学 生殖生物学
背景情况:
- 亲细胞白血病核体 (PML-NBs) 是小鼠卵细胞中缺少的核器官.
- 卵子细胞在受精和早期发育期间面临重大压力.
研究的目的:
- 调查诱导的PML-NBs对在压力下小鼠卵细胞受精的影响.
- 评估PML-NBs在胚胎发育早期的行为和清除.
主要方法:
- 将编码人类PML蛋白 (hPML VI-sfGFP) 的mRNA注入小鼠卵细胞,以诱导PML-NB的形成.
- 在卵细胞和早期胚胎中观察到PML-NB的行为,特别是在压力条件下.
- 利用基激活来研究早期胚胎发育和PML-NB清除.
主要成果:
- 诱导的PML-NBs (hmdPML-NBs) 在卵细胞细胞质中持续存在,形成碎片,特别是在压力下.
- 亲遗传胚胎从细胞质中清除了预先组装的hmdPML-NBs.
- 在发育胚胎的前核中形成了新的hmdPML-NBs.
结论:
- 没有PML-NB的核等离子体环境对卵细胞功能有益.
- 卵细胞清除细胞质物质的能力是卵细胞质量的潜在指标.
- PML-NB动态提供了对卵细胞弹性和发育能力的见解.
相关概念视频
Meiosis II
45.1K
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
45.1K
Meiosis vs. Mitosis
54.2K
Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
54.2K
Meiosis I
193.4K
Meiosis is a carefully orchestrated set of cell divisions, the goal of which—in humans—is to produce haploid sperm or eggs, each containing half the number of chromosomes present in somatic cells elsewhere in the body. Meiosis I is the first such division, and involves several key steps, among them: condensation of replicated chromosomes in diploid cells; the pairing of homologous chromosomes and their exchange of information; and finally, the separation of homologous chromosomes by...
193.4K
Oogenesis
63.5K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
63.5K


