在弱酸性条件下,通过含有H型I型脂多糖的脂多糖,通过H型I型脂多糖,通过H型I型脂多糖,通过H型脂多糖,通过H型脂多糖,通过H型脂多糖,通过H型脂多糖,通过H型脂多糖,通过H型脂多糖,通过H型脂多糖,通过H型脂多糖,通过H型脂多糖,通
Takaharu Sasaki1, Midori Oyama1, Mao Kubota1
1Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
盖लेक्ट因是有助于抵御细菌的蛋白质. 盖莱克-2和盖莱克-3与Helicobacter pylori结合,显示在不同pH条件下在胃防御中具有潜在作用.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 盖लेक्ट因是参与宿主防御的动物讲蛋白.
- 盖莱克-2和-3在胃中发现,它们向着Helicobacter pylori.
- 之前的研究表明,在中性pH下,加勒-2和3具有抗H. pylori活性.
研究的目的:
- 在弱酸性条件下研究加勒-2对H. pylori的活性.
- 在酸性pH下确定加勒-2与H.pylori脂多糖 (LPS) 的结合能力.
- 在酸性条件下比较加勒-2和3的稳定性和结合性.
主要方法:
- 循环二元化来评估蛋白质的结构稳定性.
- 亲密性染色体和西方涂抹以分析加勒-LPS结合.
- 在弱酸性条件下培养H. pylori (pH 6.0和5.0).
主要成果:
- 在pH 6.0,但不是pH 5.0,与结构稳定性相关联.
- 盖莱克-2 结合 H. pylori LPS 含有 H 型 I 抗原,以 β-银酸胺依赖的方式结合.
- 加勒-3在酸性条件下比加勒-2更稳定,并且与H型I和易斯X抗原结合H. pylori LPS.
结论:
- 盖莱克-2和3对H.pylori表现出明显的pH依赖性活动.
- 在pH值为6.0时,盖莱克-2通过结合特定的LPS结构来对抗H. pylori.
- 加勒-3在酸性条件下表现出更高的稳定性和更广泛的结合.
- 盖莱克-2和3可能在不同pH值环境中与H. pylori的胃防御合作.
更多相关视频
12:29Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility
Published on: March 11, 2022
10:44One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
Published on: June 18, 2016
相关概念视频
Gastritis-II: Pathophysiology
In acute gastritis, the gastric mucosa becomes swollen and red and undergoes superficial erosion. Superficial ulceration may lead to bleeding.
In chronic gastritis, persistent or repeated insults lead to chronic inflammatory changes and, eventually, thinning or atrophy of the gastric tissue.
Gastritis can stem from various causes, each...
Pathophysiology of Peptic Ulcer Disease: Injurious Factors
In the antrum region, G cells secrete the gastrin hormone that binds to gastrin-cholecystokinin-B (CCK2) receptors on parietal and enterochromaffin-like (ECL) cells in the fundic glands. Simultaneously, the vagus nerve releases acetylcholine, which binds...
Adherens Junctions
Adherens Junctions are Dynamic
Mucosal Barrier of the Stomach
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
Peptic Ulcer Disease II: Pathophysiology
Damaging agents such as Helicobacter pylori, gastric acid, pepsin, and nonsteroidal anti-inflammatory drugs (NSAIDs) can weaken the mucosal defense, allowing hydrogen ions to infiltrate back and harm epithelial cells.
GPCRs Regulate Adenylyl Cylase Activity
