相关实验视频

Updated: Jun 14, 2025

Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment

Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment

Published on: April 29, 2022

确定两种长非编码RNA,Kcnq1ot1和Rmst,作为老鼠慢性肝病中的生物标志物

Shinya Yokoyama¹, Hisanori Muto^1,2, Takashi Honda¹

¹Department of Gastroenterology and Hepatology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8560, Japan.

International journal of molecular sciences

|August 29, 2024

概括

两种长非编码RNA (lncRNAs),Kcnq1ot1和Rmst,在与代谢功能障碍相关的脂肪性肝脏 (MASH) 和肝纤维化的小鼠模型中显着减少,表明它们作为疾病生物标志物的潜力.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
肝病学肝病学是一种肝病学.

背景情况:

与代谢功能障碍相关的脂肪性肝 (MASH) 是一个日益严重的全球健康问题.
马什病进展为肝纤维化与预后不佳和肝癌风险增加有关.
了解MASH和肝纤维化背后的分子机制对于开发有效的治疗方法至关重要.

研究的目的:

研究MASH和小鼠肝纤维化的短寿命长非编码RNA (lncRNAs) 的作用.
为了确定在这些肝脏疾病中表达变化的特定lncRNAs.
评估已识别的 lncRNAs 作为疾病进展的生物标志物的潜力.

主要方法:

在MASH和肝纤维化小鼠模型中分析lncRNA表达.
使用西方饮食和CCl4.4诱导MASH.
使用CCl4.4诱导肝纤维化.

主要成果:

两种lncRNAs,Kcnq1ot1和Rmst,在MASH和肝纤维化模型中都被发现显著下降.
在西方饮食和CCl4引起的MASH肝脏中,Kcnq1ot1和Rmst的表达水平发生了显著的改变,在CCl4引起的纤维性肝脏中,CCl4.
与MASH相比,这些lncRNAs的减少在肝纤维化中更为明显,这表明它们作为疾病严重程度的生物标志物的实用性.

结论:

在小鼠的MASH和肝纤维化中,Kcnq1ot1和Rmst起着至关重要的作用.
这些lncRNAs可以作为监测疾病进展的有价值的生物标志物.
这些发现为探索针对MASH和肝纤维化的lncRNA向治疗提供了基础.

关键词:

这是一个很好的选择. Kcnq1ot1 第一个是RMST.肝细胞癌是肝细胞癌.在 lncRNAs 中.小鼠的肝脏是小鼠的肝脏.

更多相关视频

Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

Published on: August 27, 2020

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Published on: January 7, 2019

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...