在调节COVID-19患者NF-κB信号和细胞因子表达中的miR-9的作用
Carla Prezioso1,2, Dolores Limongi1,2, Paola Checconi1,2
1Department for the Promotion of Human Sciences and Quality of Life, San Raffaele University, Via di Val Cannuta 247, 00166 Rome, Italy.
International journal of molecular sciences
|August 29, 2024
概括
微RNA-9 (miR-9) 和NF-κB信号在严重的COVID-19中升高,特别是在未幸存者中. 向miR-9和NF-κB可能为炎症性疾病提供治疗潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 病毒学 病毒学
背景情况:
- 严重的COVID-19与细胞因子风暴和炎症有关.
- 由microRNAs调节的NF-κB信号通路与炎症反应有关.
研究的目的:
- 研究miR-9在调节NF-κB信号和COVID-19患者炎症性细胞因子表达中的作用.
- 探索miR-9作为COVID-19严重程度的潜在生物标志物.
主要方法:
- 对41名COVID-19患者和20名健康对照进行了观察后期研究.
- 用RT-PCR和ELISA测量了miR-9,NF-κB,IκBα,IL-6,IL-1β和TNF-α的水平. 这两种方法都被用于测量miR-9,NF-κB,IκBα,IL-6,IL-1β和TNF-α的水平.
- 进行了统计分析,包括相关性和回归.
主要成果:
- COVID-19 患者,尤其是未幸存者,表现出显著更高的 miR-9 和 NF-κB 水平.
- 在miR-9和NF-κB表达之间观察到强烈的正相关性 (r = 0.813,p < 0.001).
- NF-κB水平与促炎性细胞因子IL-6,IL-1β和TNF-α有很强的相关性.
结论:
- miR-9调节COVID-19中的NF-κB信号和炎症.
- 没有幸存者的miR-9水平升高表明其作为严重性生物标志物的潜力.
- 向miR-9和NF-κB可能对COVID-19和其他炎症状况具有治疗前景.
关键词:
在 COVID-19 疫情中,一个IL-1ββ.在IL-6的研究中,IL-6被认为是IL-6的基因.这就是伊克巴的意思.在 NF-κBB 中.这就是TNF-α.在 miR-9 系统中,这是一个小RNARNA.更多相关视频
13:38Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells
Published on: January 18, 2017
12.2K
09:40Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
Published on: August 2, 2024
2.6K
相关概念视频
NF-κB-dependent Signaling Pathway
7.4K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.4K
Co-activators and Co-repressors
7.3K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.3K
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
T Cell Types and Functions
963
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
963
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
The JAK-STAT Signaling Pathway
8.7K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
8.7K
