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病毒 (IHHNV) 介导的基因转移和表达系统的开发和评估

Yiwen Tao^1,2, Jinwu Wang^1,2, Rui Xiao^1,2

¹Key Laboratory of Marine Genetics and Breeding (Ministry of Education), College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.

International journal of molecular sciences

|August 29, 2024

概括

这项研究开发了一种新的病毒载体系统,用于在细胞中转移和表达基因. 该系统使用类传染性皮下和造血性亡病毒 (IHHNV),有望实现高效的基因传递.

科学领域:

水产养殖和水生动物健康
分子生物学和病毒学.
生物技术和基因工程生物技术和基因工程

背景情况:

有效的基因转移工具对于研究和水产养殖至关重要.
在和细胞中传递基因的现有方法是有限的.
开发新的病毒载体可以克服这些局限性.

研究的目的:

开发和验证DNA病毒介导的基因转移和表达系统.
使用类传染性皮下和造血性亡病毒 (IHHNV) 创建一个功能性的病毒载体.
在各种细胞和组织模型中评估系统的效率.

主要方法:

构建了一个病毒载体 (pUC19-IHHNV-PH-GUS),包含IHHNVDNA和GUS记者基因.
使用昆虫Sf9细胞进行病毒包装.
同时感染或同时感染了用于基因表达的百科病毒载体 (Bacmid或Bacmid-VP28).
在Sf9细胞,血淋巴细胞和组织中评估了基因转移和GUS表达.

主要成果:

成功地将pUC19-IHHNV-PH-GUS载体包装成Sf9细胞中的IHHNV类病毒.
在Sf9细胞,血淋巴细胞和组织中证明了基因转移效率.
由于PH促销者的Bacmid依赖性,GUS表达取决于与baculoviral载体的共同感染.
与Bacmid相比,Bacmid-VP28提高了包装和感染效率.

结论:

一种新的DNA病毒介导的基因转移系统被成功开发出来.
该系统需要与baculoviruses共同感染以实现记者基因表达.
巴克米德-VP28提高了这种基因转移系统的效率,用于的应用.

关键词:

传染性皮下和血液形成性亡病毒 (IHHNV)昆虫的Sf9细胞鱼是不是可以吃的的血淋巴细胞是的血淋巴细胞.病毒包装病毒包装病毒介导的基因转移和表达系统.

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