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转胺酶2调节HSF1基因表达在鱼眼神经再生的急性阶段

Kayo Sugitani¹, Takumi Mokuya¹, Yu Kanai¹

¹Department of Clinical Laboratory Science, Graduate School of Medical Science, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa 920-0942, Japan.

International journal of molecular sciences

|August 29, 2024

概括

转胺酶2 (TG2) 在斑马鱼的视神经受伤后迅速激活热冲击因子1 (HSF1) 和Yamanaka因子. 这种TG2介导的途径对于鱼类的急性视神经再生至关重要.

科学领域:

神经科学是一个神经科学.
再生医学是一种再生医学.
分子生物学分子生物学

背景情况:

鱼类视网膜质细胞 (RGCs) 在视神经损伤 (ONLs) 后表现出显著的再生能力.
之前的研究确定了热冲击因子1 (HSF1),Yamanaka因子,视网膜酸 (RA) 信号和转谷氨酶2 (TG2) 在ONL后的RGC存活,神经发生和神经元外生参与.

研究的目的:

研究斑马鱼视神经再生中的TG2,HSF1和Yamanaka因子之间的急性相相互作用.
阐明TG2在调节ONL后HSF1和Yamanaka因子表达中的作用.

主要方法:

使用TG2特异性的morpholino和抑制剂来阻断TG2的活性.
使用抗TG2抗体进行了ChIP测试,以评估HSF1基因组的DNA结合.
在TG2抑制后,HSF1和Yamanaka因子的量化基因表达.

主要成果:

在ONL发生后0.5小时内,TG2和RA信号增加,RA在TG2之前.
TG2抑制完全抑制了HSF1表达和Yamanaka因子基因表达.
奇普测定证实了TG2与HSF1基因组DNA的结合.

结论:

在鱼眼神经再生的早期阶段,TG2充当HSF1和Yamanaka因子的关键调节者.
TG2-HSF1信号轴对于启动斑马鱼在ONL后的再生反应至关重要.

关键词:

在HSF1中,它是HSF1.这就是Klf4 第四十四章没有了这就是Sox2x2的意思.这是TG2的TG2.亚马纳卡因素亚马纳卡因素细胞存活率细胞存活率视神经的再生和再生.视网膜视网膜视网膜是一个斑马鱼是一种斑马鱼.

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