相关实验视频
Updated: Jun 14, 2025

05:07
Characterizing Extracellular Vesicles from Biological Fluids
Published on: February 28, 2025
259
在COPD患者的唾液样本中检测和描述细胞外囊泡
Ourania S Kotsiou1, Katerina Katsanaki2, Aikaterini Tsiggene2
1Laboratory of Human Pathophysiology, Faculty of Nursing, University of Thessaly, 415 00 Larissa, Greece.
Journal of personalized medicine
|August 29, 2024
概括
细胞外囊泡 (EVs) 存在于COPD患者的唾液中,比健康个体的体积更大. 在COPD唾液中增加的EV相关蛋白质可以作为诊断标记.
科学领域:
- 肺部医学 肺部医学
- 生物标志物发现发现
- 细胞外囊泡研究研究
背景情况:
- 关于COPD患者唾液中的细胞外囊泡 (EVs) 的研究有限.
- 之前的研究并没有广泛地描述这些电动汽车.
研究的目的:
- 从COPD患者和健康对照的唾液中分离和描述EVs.
- 调查这些群体之间的 EV 特性潜在差异.
主要方法:
- 从COPD患者和健康对照组中采集了唾液样本.
- 使用膜亲和力旋转柱方法分离了EV.
- 电动汽车的特点是传输电子显微镜 (TEM) 和CD9/CD81标记者的西方斑点.
主要成果:
- 在这两组中都检测到了EV,COPD患者的平均直径更大.
- 在这两组中,EV标志物CD9和CD81都得到了丰富.
- 总的EV相关蛋白在COPD患者的唾液中显著更高.
结论:
- 在COPD患者和健康人群中都能检测到液EV.
- 慢性肺炎患者在150-200纳米范围内表现出更高的EV比率.
- 在COPD唾液中增加了EV相关蛋白质,这表明它有可能作为诊断生物标志物.
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