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对23种基因多态与基因多态的关联分析基因多态与基因多态与基因多态具有生殖特征基因多态与基因多态具有生殖特征基因多态与基因多态具有生殖特征

Jie Sun^1,2, Chunyuan Wang^1,2, Yan Wu^1,2

¹Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, West Campus, Huaxi District, Guiyang 550025, China.

|August 29, 2024

概括

在Kele猪中调查甲基转移酶样23 (METTL23) 基因单核酸多态性,发现与生殖特征有显著的关联. 特定的METTL23变异增强了 litter 尺寸和重量,这表明它有可能用于繁殖计划.

科学领域:

动物遗传学和动物繁殖
分子生物学分子生物学
生殖生物学生殖生物学

背景情况:

甲基转移酶样23 (METTL23) 是一种RNA甲基转移酶,参与关键的生物过程.
猪中METTL23的遗传作用,特别是关于生殖表现的遗传作用,仍未得到充分研究.
了解METTL23中的遗传变异可以为提高猪生殖效率提供见解.

研究的目的:

研究METTL23基因中单核酸多态性 (SNPs) 对Kele猪生殖特征的遗传影响.
为潜在的育种应用确定与增强生殖性能相关的特定METTL23变异.

主要方法:

从228只多的Kele母猪中提取DNA.
桑格测序用于识别METTL23基因中的三个SNP:g.4804958 G > T (intron 2),g.4805082 C > T (外显子2) 和g.4806821 A > G (外显子3).
多态信息内容 (PIC) 计算,链接不平衡分析和与生殖特征的关联分析.

主要成果:

确定了三种SNP (g.4804958 G > T,g.4805082 C > T,g.4806821 A > G),表现出中度多态.
SNP g.4804958 G > T:GG基因型与显著更高的活生生的小猪, litter出生体重,断奶的小猪和断奶 litter 体重相关 (p < 0.05).
SNP g.4806821 A > G:GG基因型与明显更高的 litter 出生体重和平均出生体重相关 (p < 0.05). H4H4双型显著影响了多种生殖特征 (p < 0.05).

结论:

METTL23基因含有显著的遗传变异,影响Kele猪的生殖特征.
特定的METTL23SNP的基因型和双型与改善的生殖性能有关,包括幼大小和体重.
METTL23可以被认为是一个有价值的候选基因,用于标记器辅助的选择,以增强Kele猪繁殖计划中的生殖特征.

关键词:

凯莱猪是个猪.在METTL23中,使用的是METTL23 生育特征的生育特征一个核酸的多态性.

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