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在精英长途跑步者最大耐力测试的反应中,循环miRNA对脂肪酸代谢的信号传递

Dailson Paulucio¹, Carlos Ramirez-Sanchez¹, Rodolfo Velasque¹

¹Biometrics Laboratory (LADEBIO), Federal University of Rio de Janeiro, Rio de Janeiro 21941-599, Brazil.

|August 29, 2024

概括

表观遗传学,特别是微RNAs (miRNAs),影响精英运动员的最大氧气摄入量 (VO2max). 这些miRNA显著准脂肪酸代谢途径,突出显示它们在耐力表现中的作用.

科学领域:

运动生理学运动生理学
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

最大氧吸收量 (VO2max) 是耐力运动员心肺健康的关键指标.
表观遗传因素,特别是microRNAs (miRNAs),在VO2max水平和变化方面发挥着重要作用.
精英耐力运动员表现出不同的VO2max水平,尽管训练量相似,这表明潜在的遗传和表观遗传影响.

研究的目的:

为了研究精英耐力运动员具有不同VO2max水平的血miRNA概况.
通过急性最大耐力测试来识别特定的miRNA及其相关的生物学途径.
在训练有素的运动员中探索miRNA表达,VO2max和脂肪酸代谢之间的关系.

主要方法:

从23名精英耐力运动员那里收集了血样本,在进行最大分级运动测试之前和之后.
六名运动员被分为高 (75.4mL.kg-1.min-1) 和低 (60.1mL.kg-1.min-1) VO2max组.
提取了RNA,并使用1113个miRNART-qPCR面板来分析差异性miRNA表达.
对显著表达的miRNA进行了生物信息分析 (DIANA工具),以确定向途径.

主要成果:

发现51个miRNA在高和低VO2max组之间表达不同.
基于性能水平和时间点 (运动前与运动后) 聚合的miRNA表达模式.
脂肪酸代谢途径在所有组和时间点都被差异表达的miRNA显著准 (p < 0.001).

结论:

精英耐力运动员之间具有高和低VO2max的血miRNA配置文件有所不同.
通过miRNAs的表观遗传调节,特别是那些参与脂肪酸代谢的人,有助于心肺呼吸能力.
这些发现表明,精英运动员的遗传/表观遗传因素,脂肪酸通路和耐力表现之间存在联系.

关键词:

耐力精英运动员耐力精英运动员运动表现运动表现脂肪酸代谢脂肪酸代谢最大的氧气吸收.这是一个小RNARNA.

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