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转录组分析揭示了新型炎症信号对Glaesserella parasuis的感染

Jingwen Lei^1,2, Xuexue Chen^1,2, Huanhuan Zhou^1,2

¹Laboratory of Genetic Breeding, Reproduction and Precision Livestock Farming, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, China.

|August 29, 2024

概括

格拉塞雷拉寄生虫导致严重的猪炎症和经济损失. 研究人员确定了参与炎症反应的关键基因和途径,为疾病耐药性繁殖铺平了道路.

科学领域:

兽医免疫学兽医免疫学
分子生物学分子生物学
猪病理学猪病理学

背景情况:

玻璃虫 (Glaesserella parasuis,GPS) 感染导致猪严重的全身炎症,造成严重的经济损失.
目前用于预防和控制GPS感染的方法是无效的,这凸显了对抗病策略的需要.
识别抗病基因对于猪的分子繁殖至关重要.

研究的目的:

调查猪体内由Glaesserella parasuis感染引起的全身急性炎症背后的分子机制.
建立体外感染模型,用于研究GPS病原体.
识别潜在的抗病基因和参与宿主对GPS反应的信号通路.

主要方法:

使用3D4/21,PK15和PAVEC细胞建立了三种体外感染模型,以模仿GPS感染.
在GPS刺激后分析了细胞反应,包括细胞亡和炎症因子上调.
进行了转录组分析以识别差异表达基因 (DEGs),并利用英才途径分析 (IPA) 进行途径丰富和受体识别.

主要成果:

在所有三种细胞模型中,GPS感染显著提高了炎症因子的调节,而在细胞亡中没有显著差异.
转录组分析揭示了许多与炎症反应,细胞增殖和基因表达相关的DEG.
IPA确定了关键的信号通路 (S100家族,病原体识别,细胞因子风暴) 和潜在的受体 (F3,ICAM1,PLAUR,ACKR3,GPRC5A),其中ACKR3和GPRC5A因其在免疫反应中的作用而被突出.

结论:

这项研究阐明了由猪GPS感染引起的全身炎症的病理机制.
确定了关键基因,信号通路和参与宿主对GPS炎症反应的受体.
为未来关于对猪GPS感染的抗病育种的研究提供了基础.

关键词:

玻璃虫 (Glaesserella parasuis) 已经被发现.细胞响应的细胞反应.具有抗病能力的繁殖.系统性炎症反应的系统性炎症反应转录基因组测序的转录基因组测序

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