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在酶提取,净化和生物转化水性两相系统的进步

Nikša Bekavac¹, Maja Benković¹, Tamara Jurina¹

¹Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia.

Molecules (Basel, Switzerland)

|August 29, 2024

概括

水性双相系统 (ATPS) 为传统的液体液体提取提供了可持续的替代方案,利用绿色溶剂进行高效的生物分子分离. 这种方法可以提高纯度和产量,同时尽量减少工业过程对环境的影响.

科学领域:

绿色化学和生物技术的发展
分离科学分离科学
生物化学工程生物化学工程

背景情况:

传统的液体-液体提取 (LLE) 依赖于有毒溶剂,造成环境和健康风险.
对可持续工业工艺的日益增长的需求迫使人们探索环保的分离方法.
水性双相系统 (ATPS) 成为部件分离的有希望的绿色替代方案.

研究的目的:

审查ATPS的原则,应用和优势,作为传统LLE的可持续替代方案.
探索ATPS的热力学基础和影响因素,特别是在酶提取方面.
突出ATPS在工业生物转化过程中整合的进步和挑战.

主要方法:

对管理ATPS的热力学原理的审查.
在ATPS中影响酶分化的因素分析.
探索最近的进步,包括在ATPS中使用离子液体和深层环氧溶剂.
讨论生物转化ATPS应用中的挑战.

主要成果:

ATPS提供高纯度和单步提取生物分子.
使用特定组件,如离子液体和深溶解剂,可以提高ATPS的效率.
ATPS显示了提高酶提取产量和纯度的潜力.
在酶稳定性和工业集成过程优化方面仍然存在挑战.

结论:

对于各种工业分离工艺,特别是生物技术,ATPS是一种可行和可持续的技术.
对酶稳定性和过程优化的进一步研究对于ATPS在工业上广泛采用至关重要.
ATPS为更绿色,更有效的生物转化和生物分子净化提供了显著的潜力.

关键词:

水性双相系统 (ATPS) 是一种水性双相系统.生物转化过程中的生物转化.酵素提取酶的提取方法绿色的溶剂绿色的溶剂液体液体提取 (LLE) 的方法

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