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通过修改的四酶合成生物系统从乙醇中有效地生产2,3-butanediol

Jiming Zhang¹, Hui Lin², Chaosong Zheng³

¹College of Chemical Engineering, Huaqiao University, Xiamen 361021, China.

Molecules (Basel, Switzerland)

|August 29, 2024

概括

工程形式酶 (FLS) 从乙醇中显著增加了2,3-butanediol (2,3-BD) 的产生. 这种增强的酶和无细胞系统实现了可持续化学制造的高产量和生产率.

科学领域:

生物催化剂和代谢工程
合成生物学用于化学生产.
可持续化学和生物基化学品

背景情况:

2,3-butanediol (2,3-BD) 是一种有价值的生物基平台化学物质.
之前的工作建立了四个酶系统,用于乙醇转化为2,3-BD.
甲酶 (FLS) 的催化效率较低,限制了系统的性能.

研究的目的:

为了提高催化效率,设计形式酶 (FLS).
开发一个增强的无细胞生物系统用于2,3-BD生产.
通过酶催化,从乙醇中优化2,3-BD的合成.

主要方法:

通过修改其道和基质结合口袋来改造FLS的蛋白质工程.
创建单点和组合式FLS变体.
实现一个无细胞生物系统,利用工程FLS (FLS:I28V/L482E) 进行乙醇升级.

主要成果:

与野生类型相比,设计的FLS变体显示出更高的催化效率和基板耐受性.
使用FLS:I28V/L482E的无细胞生物系统实现了2.3-BD度为1.39M (124.83g/L).
高生产率 (5.94 g/L/h) 和产量 (92.7%) 通过料批发酵获得.

结论:

FLS的酶工程有效地提高了催化性能.
修改后的无细胞酶系统为可持续的2,3-BD生产提供了一个有希望的途径.
这种方法为工业2,3-BD制造提供了具有成本竞争力的替代方案.

关键词:

催化性能催化性能催化性能没有细胞的多酶催化剂.乙醇提升升级乙醇提升升级一个半理性的设计.在基板的耐受性.

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