来自Puccinia triticina的Effector Pt9226在小麦系TcLr15中具有病毒性作用

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Bingxue Wang¹, Jiaying Chang¹, Johannes Mapuranga¹

¹Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province, Hebei Agricultural University, Baoding 071000, China.

|August 29, 2024

概括

来自Puccinia triticina (Pt) 的效应蛋白Pt9226作为一种毒性因子,抑制编程细胞死亡 (PCD) 并抑制小麦中的植物防御反应. 这项研究描述了Pt9226的特征.

科学领域:

植物病理学植物病理学
分子植物-微生物相互作用
遗传学是一个遗传学.

背景情况:

病原体作用因子是宿主-病原体相互作用中的关键毒性因素.
普奇尼亚三 (Pt) 是小麦的重要病原体,需要对其毒性机制进行研究.

研究的目的:

为了描述来自Puccinia triticina (Pt) 病变型THTT.的新型效应体候选物Pt9226的特征.
研究Pt9226在宿主-病原体相互作用中的作用及其对植物防御机制的影响.

主要方法:

克隆和对Pt9226效应基因的序列分析.
生物信息学分析用于同类学和功能预测.
用RT-qPCR分析Pt9226在注射后的表达模式.
使用GFP融合蛋白进行亚细胞局部化研究.
功能性试验评估Pt9226对烟草和小麦编程细胞死亡 (PCD) 和植物防御反应的影响.

主要成果:

克隆和鉴定了pt9226的特征,显示了与其他假定效应蛋白质的同质性.
在Pt注射后显著诱导Pt9226表达,在注射后36小时达到峰值 (hpi).
烟草中Pt9226抑制的BAX/INF1诱导的PCD和小麦中DC3000诱导的PCD.
Pt9226的短暂表达抑制了小麦中的和ROS积累,并调节了与病变发生相关的基因 (TaPR1,TaPR2,TaTLP1) 的表达.

结论:

在Puccinia triticina病变型THTT和小麦之间的相互作用中,Pt9226作为毒性作用因子.
Pt9226可能通过抑制宿主防御反应,包括PCD和与防御相关基因的表达来促进病原体的毒性.

关键词:

普奇尼亚 (Puccinia triticina) 是一种的植物.在Triticum aestivum中,它是最重要的.效应器效应器是一个有效的效应器.过渡性表达的过渡性表达毒性毒性是一种毒性.

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