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Mostafa Naguib^1,2, Shreeya Sharma¹, Abigail Schneider¹

¹Department of Animal and Veterinary Science, Clemson University, Clemson, SC 29634, USA.

|August 29, 2024

概括

用Campylobacter jejuni lysate或外膜蛋白 (OMP) 进行皮下接种,特别是用CpG ODN,有效地减少了的Campylobacter殖民. 在卵内接种疫苗没有显示出任何保护作用.

科学领域:

兽医免疫学兽医免疫学
疫苗的研发工作正在进行中.
食品安全食品安全

背景情况:

全球范围内,Campylobacter jejuni是导致细菌性胃肠炎的主要原因.
禽类是C. jejuni的主要储存库,构成严重的食品安全风险.
需要有效的疫苗来控制的坎皮洛巴克特菌殖民.

研究的目的:

评估和比较不同C. jejuni疫苗配方的疗效.
评估免疫刺激剂CpG ODN在疫苗疗效中的作用.
为了确定C. jejuni疫苗的最佳注射途径 (卵内 vs. 皮下注射).

主要方法:

进行了两项试验,涉及不同的C. jejuni抗原 (热不活化,全溶解体,外膜蛋白 (OMP)) 和CpG ODN.
疫苗可以在卵子中给胚胎,也可以在一天大的小下进行皮下注射 (SC).
测量了免疫反应 (细胞因子基因表达,抗体水平) 和Cecal C. jejuni殖民.

主要成果:

用C. jejuniOMP与CpGODN相结合的SC免疫显著增加了免疫标记物 (IFN-γ,IL-1β,IL-13,IgM,IgY) 并减少了CECAL C. jejuni数量1.2 log10.
在卵子中接种疫苗并没有诱导免疫反应或保护.
对C. jejuni全溶解酸或200μg的OMP进行下注,C. jejuni的数量分别降低了1.4和1.1log10.

结论:

C. jejuni全溶解酸和OMPs是有希望的疫苗抗原,可以减少的坎皮洛巴克特菌殖民.
在诱导保护性免疫力方面,皮下施用比 in ovo 更有效.
CpG ODN可以提高C. jejuni OMP疫苗的疗效.

关键词:

这是坎比洛巴克特菌.在CpG ODN中使用.这些都是,,.在卵子里外膜蛋白质是外膜中的一种蛋白质.疫苗疫苗疫苗疫苗

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