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波兰马群中马类鼻炎病毒的流行率和序列分析
Karol Stasiak1, Magdalena Dunowska2, Jerzy Rola1
1Department of Virology, National Veterinary Research Institute, 24-100 Pulawy, Poland.
Viruses
|August 29, 2024
概括
马鼻炎B病毒 (ERBV) 在1.78%的波兰马匹中被检测到,这标志着其在该国首次被发现. 基因分析揭示了与个人马匹和马匹相关的变异性.
科学领域:
- 兽医病毒学 兽医病毒学
- 马类传染病 马类传染病
- 分子流行病学分子流行病学
背景情况:
- 马鼻炎A (ERAV) 和B (ERBV) 是全球普遍存在的呼吸道病毒,影响马.
- 了解它们的流行率和遗传多样性对于疾病管理至关重要.
研究的目的:
- 为了确定波兰马中ERAV和ERBV感染的频率.
- 分析检测到的ERBV菌株的遗传变异性.
- 在波兰报告首次检测到ERBV.
主要方法:
- 量化RT-PCR测定被用来选来自16个波兰国家研究中的621匹马的鼻子拭子.
- 阳性样本经历了部分DNA聚合酶基因放大和测序.
- 在获得的序列上进行了遗传学和遗传网络分析.
主要成果:
- 在任何样本中都没有检测到ERAV.
- 在621匹 (1.78%) 的马中,ERBV被确定在11匹,包括健康的个人和一个有呼吸道征兆的小马.
- ERBV DNA聚合酶基因的序列变异性与波兰境内个体马匹和马匹相关.
- 当包括国际序列时,ERBV序列和原产国之间没有发现相关性.
结论:
- 这项研究报告了波兰首次检测到马鼻炎B病毒.
- 波兰马中发生ERBV感染,影响健康的动物和患有呼吸系统疾病的动物.
- 波兰境内ERBV的局部遗传变异性表明,可能存在不同的流行病学模式.
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