在黑兵中检测出已知和新型病毒序列,并表达宿主抗病毒途径
Hunter K Walt1, Heather R Jordan2, Florencia Meyer1
1Department of Biochemistry, Nutrition and Health Promotion, Mississippi State University, Mississippi State, MS 39762, USA.
Viruses
|August 29, 2024
概括
研究人员在黑兵中发现了两种新病毒,影响了昆虫养殖. 这项研究确定了新型病毒,并探索它们与宿主昆虫的相互作用,这对于可持续的蛋白质生产至关重要.
科学领域:
- 病毒学 病毒学
- 昆虫学 昆虫学是一门学科.
- 昆虫病理学 昆虫病理学
背景情况:
- 像黑兵 (Hermetia illucens) 这样的昆虫的大规模养对于可持续的蛋白质生产至关重要.
- 密切的养殖条件可以促进病原体的传播,对工业昆虫种群构成风险.
- 此前只发现了三种与黑兵相关的病毒,这突显了知识上的差距.
研究的目的:
- 为了在黑兵中识别新型病毒,使用metatranscriptomic测序.
- 为了描述黑士兵内病毒的多样性,肠道,,和饮食.
- 调查潜在的宿主病毒相互作用及其对黑兵生产的影响.
主要方法:
- 黑兵样本的元转录组测序 (肠道,,饮食).
- 遗传学分析以确定病毒关系.
- 对宿主基因表达的分析,以确定抗病毒反应.
主要成果:
- 检测了两种新型病毒:Hermetia illucens sigma样病毒1和Hermetia illucens昆虫样病毒1.
- 识别Hermetia illucens类似昆虫的病毒1作为一种新的昆虫病毒家族 (昆虫病毒).
- 确认先前确定的病毒 (BSF奈洛样病毒1,BSF无特征的布尼亚病毒样1) 和检测在抗病毒途径中的差异性基因表达.
结论:
- 这项研究扩大了黑兵已知的病毒组,发现了两种新型病毒.
- 黑兵病毒相互作用活跃,有宿主抗病毒反应的证据.
- 这些发现对于理解和管理黑兵养殖中潜在的病毒威胁具有重要意义.
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