用于Arbovirus监测的基因组学:公共卫生实验室常规使用的考虑因素
Leandro Patiño1,2, Andrea Denisse Benítez3, Andrés Carrazco-Montalvo4
1Instituto Nacional de Investigación en Salud Pública "Leopoldo Izquieta Pérez", Dirección Técnica de Investigación, Desarrollo e Innovación, Guayaquil 090150, Ecuador.
Viruses
|August 29, 2024
概括
高通量测序 (HTS) 为关节动物传播的病毒 (arbovirus) 监测提供了具有成本效益的方法. 本综述详细介绍了HTS对可重复性公共卫生实验室分析的技术考虑.
科学领域:
- 病毒学 病毒学
- 公共卫生 公共卫生
- 基因组学就是基因组学.
背景情况:
- 关节动物传播的病毒 (arboviruses) 由于它们的出现和重新出现,造成了严重的公共卫生风险.
- 公共卫生实验室的有效监测需要具有成本效益和可重复性的方法.
研究的目的:
- 审查国家卫生实验室对 arbovirus 监测的高通量测序 (HTS) 的技术考虑.
- 为 arboviruses 的测序方法和生物信息学管道的当前进步提供见解.
主要方法:
- 专注于HTS工作流中的序列化前,序列化和序列化后方法.
- 强调强大的湿干实验室协议对于可重复性分析的重要性.
主要成果:
- 讨论与 arbovirus 检测和表征相关的 HTS 的技术方面.
- 强调对测序方法和生物信息学进行监控的整合.
结论:
- 在公共卫生机构中,HTS提供了一种可行和可重复的方法,用于公共卫生机构的树冠病毒监测.
- 本综述为研究人员和临床医生提供了一份关于实施HTS用于 arbovirus 诊断和监测的指南.
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