长非编码RNAMALAT1通过转基因细胞的转基因分化来调解纤维状地形驱动的病理化
Woo-Jin Kim1, Jieun Bae1, Eun-Hye Lee1
1Department of Molecular Genetics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, 08826, Republic of Korea.
Materials today. Bio
|August 29, 2024
概括
生物材料纤维基质可以通过调节长非编码RNA Malat1来触发细胞化. 这一发现为预防医疗植入物中病态化提供了新的策略.
科学领域:
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 假肢诱导的病态化是重建医学中的一个主要并发症.
- 纤维细胞外基质 (ECM) 在触发生物材料附近细胞骨质性转基因差异化的作用尚不清楚.
研究的目的:
- 调查仿制生物材料的纤维基质能否诱导肌细胞中的骨质性转基因分化.
- 阐明这一过程背后的分子机制,包括马拉特1的参与.
主要方法:
- 设计了一种异质聚钢纤维基质 (PSF) 来模仿ECM.
- 在PSF上培养了C2C12核细胞,并评估了体外和体内的矿物化.
- 进行了转录基因分析,以识别关键的调节分子,如马拉特1.
- 利用基因沉默技术来研究Malat1和Yap1信号的功能.
主要成果:
- 在PSF上培养的髓母细胞表现出骨质性质和矿化.
- 长非编码RNA Malat1的升调在PSF上的肌细胞细胞中被观察到.
- 沉默马拉特1抑制了PSF诱导的矿化,并降低了骨形态遗传蛋白 (Bmps) 和骨质原生标记的调节.
- PSF激活了Yap1信号,并诱导了马拉特1促进体中的表观遗传修饰.
结论:
- 与生物材料相邻的纤维矩阵可以诱导马拉特1的上调,驱动肌细胞骨质转基因差异化和子宫外化.
- 马拉特1调节骨质基因,包括Bmps,这表明了病理性化的新机制.
- 针对马拉特1是一个潜在的治疗策略,可以缓解假肢诱导的化.
相关概念视频
Formation of Muscle Fibers from Myoblasts
4.8K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
4.8K
lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
Satellite Stem Cells and Muscular Dystrophy
1.9K
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
1.9K
Forced Transdifferentiation
1.9K
Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial...
Artificial...
1.9K
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K


