杆菌的抗菌耐药性和下一代测序的作用
Christine B Andrews1, Michael I Herzlinger1,2, Steven F Moss2,3
1Division of Pediatric Gastroenterology, Nutrition, and Liver Diseases, Hasbro Children's Hospital, Providence, Rhode Island.
Gastroenterology & hepatology
|August 29, 2024
概括
抗生素耐药的Helicobacter pylori (H. pylori) 感染正在增加,使标准治疗变得不那么有效. 下一代测序 (NGS) 为个性化H. pylori治疗和监测提供了一个有希望的解决方案.
科学领域:
- 医学微生物学 医学微生物学
- 传染性疾病 传染性疾病
- 基因组学和个性化医学
背景情况:
- 杆菌 (H. pylori) 感染是一个全球性的健康问题,与胃潰瘍和胃癌有关.
- 增加H. pylori菌株的抗生素耐药性降低了标准根除疗法的有效性.
- 目前的治疗策略,包括经验方法,显示成人和儿童的成功率下降.
研究的目的:
- 审查美国H. pylori的抗菌素耐药性模式.
- 为下一代测序 (NGS) 在H.pylori管理中的临床实用性提供指导.
- 探索NGS如何个性化治疗和协助流行病监测.
主要方法:
- 对H. pylori的抗菌素耐药性模式的文献综述.
- 分析下一代测序 (NGS) 在临床实践中的作用和潜力.
- 检查NGS识别耐药性和易感性的遗传生物标志物的能力.
主要成果:
- 低于最佳的根除率凸显了需要替代H. pylori管理策略的需要.
- 基于培养的H. pylori敏感性测试未得到充分利用,并且难以实施.
- NGS成为预测抗微生物耐药性和易感性模式的可行替代方案.
结论:
- 通过启用定制方案,NGS技术具有改变H. pylori治疗的巨大潜力.
- 将NGS纳入常规临床实践可以改善患者的治疗结果,并支持公共卫生监测.
- 在NGS可访问性方面的进步对于其在管理H. pylori感染中的广泛采用至关重要.
相关概念视频
01:16Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy
335
Helicobacter pylori, a resilient gram-negative bacterium, can thrive in the stomach's harsh, acidic environment. Infection with H. pylori leads to a cascade of events within the stomach lining. One of the critical disruptions caused by this bacterium is the interference with somatostatin production, a hormone responsible for regulating acid secretion. This interference tips the balance, escalating acid secretion and diminishing bicarbonate levels. This imbalance compromises the defensive...
335
03:00Next-generation Sequencing
88.5K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.5K
00:57Antibiotic Selection
52.4K
Overview
52.4K
00:46Genomic DNA in Prokaryotes
43.6K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
43.6K