相关实验视频

Updated: Jun 14, 2025

Manufacture and Drug Delivery Applications of Silk Nanoparticles

Manufacture and Drug Delivery Applications of Silk Nanoparticles

Published on: October 8, 2016

专纳米囊和纳米晶体,用于有效的细胞内药物释放

Sharafudheen Pottanam Chali¹, Jaana Westmeier², Franziska Krebs³

¹Max Planck Institute for Polymer Research, Mainz, Germany. landfest@mpip-mainz.mpg.de.

Nanoscale horizons

|August 29, 2024

概括

开发了两种新的蛋白涂层纳米载体,用于向药物输送. 与纳米囊相比,纳米晶体显示出更高的细胞内药物释放和减少毒性.

科学领域:

纳米技术纳米技术
药理学药理学是指药理学的学科.
细胞生物学细胞生物学

背景情况:

针对特定细胞区的向药物输送对于治疗疗效至关重要.
纳米级药物输送系统可以提高循环时间并减少系统毒性.
阿托瓦 (Ato) 是一种抗原动物药物和STAT3抑制剂,是一种疏水性小分子,需要有效的输送.

研究的目的:

开发和表征两个用于细胞内药物输送的蛋白涂层纳米载体.
使用内置染料跟踪纳米载体的细胞吸收和细胞内酶开放.
评估通过纳米载体输送的阿托瓦昆的疗效和毒性.

主要方法:

开发的卵胺涂层纳米囊 (Ato-nCap) 和人类血清胺涂层纳米晶体 (Ato-nCry) 装有阿托瓦.
使用内置染料,跟踪各种人体免疫细胞类型的细胞吸收.
评估人类树突细胞和黑色素瘤细胞中的STAT3酸化抑制,以确定细胞内药物释放和疗效.

主要成果:

阿托-nCap优先进入人类髓状细胞.
Ato-nCry在所有免疫细胞类型中分发了货物,包括T和B细胞.
从Ato-nCry中释放的细胞内阿托瓦奎比自由的阿托瓦奎更有效,毒性更小.

结论:

白蛋白涂层的纳米载体是细胞内药物输送的有效系统.
纳米晶体配方显示出优异的细胞内药物释放和降低毒性.
这些纳米载体为用疏水药物向治疗提供了一个有前途的方法.

更多相关视频

Formulation and Characterization of Bioactive Agent Containing Nanodisks

Formulation and Characterization of Bioactive Agent Containing Nanodisks

Published on: March 17, 2023

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier

Published on: February 8, 2017

相关概念视频

Drug Distribution: Tissue Binding

Drug Distribution: Tissue Binding

Upon entering the systemic circulation, drugs can distribute into the interstitial and intracellular fluid of various tissue cells. This distribution is facilitated by the binding of drugs to different cellular components within tissues, which may lead to drug accumulation in specific areas. Drugs bound to tissue components serve as reservoirs that release free drugs back into the system, prolonging the drug's overall action. However, this accumulation can also result in local toxicity.
For...

Drug Distribution: Plasma Protein Binding

Drug Distribution: Plasma Protein Binding

Drugs predominantly attach to plasma proteins, with only a small percentage remaining unbound. The unbound portion can be calculated as one minus the bound fraction. Acidic drugs form large, inactive complexes by reversibly binding to plasma albumin, which prevents them from diffusing across biological barriers. These drug-protein complexes act as reservoirs for the drugs. As the concentration of unbound drugs decreases, these complexes quickly dissociate to release the free drug, maintaining...