Circ_0076490沉默抑制MAPK1表达以减少Jurkat细胞的增殖和增加Jurkat细胞的亡,通过调节miR-144-3p在严重肌痛性肌痛症
Qin Ye1, Chengyao Gu2, Wang Yan2
1Department of Pulmonary, Ningbo No.2 Hospital, Ningbo, China.
Neurological research
|August 29, 2024
概括
循环RNAcirc_0076490在严重肌痛性肌痛症 (MG) 中被上调,并通过抑制miR-144-3p来驱动疾病的进展,这反过来又激活了MAPK1. 准circ_0076490为MG提供了一个潜在的治疗策略.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 骨髓灰质炎 (MG) 是一种影响神经肌肉结合的自身免疫性疾病.
- MG的发病过程涉及循环RNAs (circRNAs),但circ_0076490的具体作用尚不清楚.
研究的目的:
- 为了研究circ_0076490在肌痛性骨髓灰质炎中的作用.
- 阐明涉及miR-144-3p和MAPK1.1的潜在分子机制.
主要方法:
- 定量的实时PCR测量RNA水平.
- 细胞增殖,细胞循环和细胞亡测定 (CCK-8,EDU,流细胞计).
- 对RNA相互作用的生物信息预测和实验验证 (双化酶,RNA下拉).
主要成果:
- 在MG患者中,Circ_0076490和MAPK1表达升高,而miR-144-3p降低.
- Circ_0076490 Knockdown 抑制了 Jurkat 细胞的增殖,并促进了细胞亡.
- Circ_0076490的目标是miR-144-3p,它的目标是MAPK1,形成了一个监管轴.
结论:
- 通过miR-144-3p/MAPK1通路,Circ_0076490 Knockdown抑制了Jurkat细胞的增殖并诱导了Jurkat细胞的亡.
- 这个circ_0076490/miR-144-3p/MAPK1轴代表了肌痛性骨髓灰质炎的潜在治疗标.
相关概念视频
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
01:07MAPK Signaling Cascades
5.3K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.3K
01:20The JAK-STAT Signaling Pathway
8.7K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
8.7K
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
01:19Interactions Between Signaling Pathways
6.2K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.2K
02:34Inhibition of Cdk Activity
4.7K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.7K