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相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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通过极化多重复合的LED照明进行单次射击强度衍射断层扫描.

Chuanjian Zheng, Shaohui Zhang, Delong Yang

    Optics letters
    |August 29, 2024
    PubMed
    概括

    这项研究引入了一种快速的3D成像技术,使用偏光来详细可视化生物样本. 该方法实现了高分辨率,允许精确分析细胞和组织中的复杂结构.

    科学领域:

    • 光学和光子学 在光学和光子学.
    • 生物医学成像技术 生物医学成像技术
    • 计算显微镜的使用

    背景情况:

    • 精确的生物样本3D重建对于理解细胞结构和功能至关重要.
    • 传统的断层扫描方法可能很慢,需要多次测量,限制了它们在动态生物过程中的应用.
    • 开发快速,高分辨率的3D成像技术对于推动生物医学研究至关重要.

    研究的目的:

    • 介绍一种用于快速3D成像的新型单射强度衍射断层扫描方法.
    • 展示开发的方法用于成像各种生物医学样本的能力.
    • 在横向和轴向尺寸中实现高空间分辨率,以进行详细的样本重建.

    主要方法:

    • 采用三种不同的线性偏光角度 (0°,45°,135°) 的极化多重 LED 照明,同时照明样品.
    • 在单个强度图像中记录散射光场,使用偏振传感器.
    • 分离单个照明贡献并执行切片式解卷,以重建3D复杂折射率分布.

    主要成果:

    • 从单次射击强度衍射数据成功重建了3D复杂的折射率分布.
    • 实现了接近不连贯的偏光限制的690nm的侧面分辨率.
    • 获得了4.68微米的轴分辨率.

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    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
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    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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  • 证明了美国空军吸收分辨率目标,大鼠海马细胞系和海绵状的有效成像.

    • 结论:

    • 本文介绍的单次射击强度衍射断层扫描方法为各种生物医学样本的3D成像提供了快速有效的方法.
    • 该技术提供了高分辨率,适用于细胞和组织结构的详细形态分析.
    • 这种方法有可能用于生物研究和诊断领域的应用,这些领域需要快速,高准确度的3D成像.