植物性大麻素和烯在体外炎症Tregs和Th17细胞上的抗炎作用
Kyle B C Tan1, H Denis Alexander1, James Linden2
1Personalised Medicine Centre, School of Medicine, Ulster University, C-TRIC Building, Londonderry BT47 6SB, United Kingdom.
Experimental and molecular pathology
|August 29, 2024
概括
大麻和四大麻显示出降低炎症反应的潜力. 这些大麻化合物在体外模型中显著抑制了促炎和抗炎细胞因子分泌,表明了免疫调节作用.
科学领域:
- 免疫学 免疫学 免疫学
- 药理学 药理学是指药理学的学科.
- 分子生物学分子生物学
背景情况:
- 大麻sativa化合物,包括植物性大麻素和烯,已经显示出有限的抗炎和止痛特性.
- 这些化合物的免疫调节潜力,特别是减少炎症性细胞因子表达和激活,需要进一步研究.
研究的目的:
- 为了测试植物大麻素在体外具有免疫调节作用的假设.
- 研究植物大麻素对炎症性细胞因子表达和免疫细胞激活的影响.
主要方法:
- 来自健康捐赠者的外周血液单核细胞 (PBMC) 被激活并用各种植物性大麻素和烯进行治疗.
- 流细胞计用于评估调节性T细胞 (Treg) 和T辅助17 (Th17) 细胞的反应.
- 使用定量实时PCR (qRT-PCR) 和ELISA分析细胞因子和炎症标记物的基因表达,并量化细胞因子分泌.
主要成果:
- 卡纳比杰罗尔,卡里奥菲伦氧化物和玛烯降低了关键炎症标记物的细胞毒性和基因表达 (IL6,IL10,TNF,TRPV1,CNR1,HTR1A,FOXP3,RORC,NFKB1).
- 四大麻素抑制了T细胞的激活,降低了RORC和NFKΒ1基因表达的调节,并减少了IL-6和IL-10的分泌.
- 大麻二醇显著抑制了Treg和Th17细胞的激活,并以剂量依赖的方式降低了IL-6和IL-10的分泌.
结论:
- 这项研究提供了第一个证据,表明大麻和四大麻可以抑制抗炎和促炎细胞因子的细胞外表达.
- 这些发现突显了特定植物性大麻素在体外PBMC模型中作为炎症反应调节者的潜力.
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