功能多态度调节FOXO1转录表达,并有助于HDM诱导过敏性鼻炎的风险和症状严重程度
Yang Yie Sio1, Kefan Du2, Terence Yin Weng Lam2
1Department of Biological Sciences, National University of Singapore, Singapore, Singapore, dbssioy@nus.edu.sg.
International archives of allergy and immunology
|August 29, 2024
概括
福克索1的遗传变异与过敏性鼻炎 (AR) 风险和症状严重程度有关. 一种特定的SNP (rs9549246) 影响FOXO1的表达,增加了受研究的人群中的AR易感性.
科学领域:
- 免疫遗传学 免疫遗传学
- 过敏性炎症研究研究
- 分子生物学分子生物学
背景情况:
- 叉头盒蛋白O1 (FOXO1) 在过敏炎症中对免疫调节至关重要.
- 关于过敏性鼻炎 (AR) 病原发生的FOXO1表达的遗传变异的作用尚未完全理解.
研究的目的:
- 研究FOXO1单核酸多态 (SNPs) 对AR发展的功能影响.
- 分析FOXO1表达,AR表型和遗传变异之间的关联.
主要方法:
- 横截面遗传和流行病学研究 (SMCSGES) 涉及5072人.
- 评估了周围血液单核细胞 (PBMC) 中的FOXO1转录水平,并与AR,全鼻症状得分 (TNSS) 和SNP基因型相关.
- 使用体外促进剂光酶试验来确定与AR相关的SNP对FOXO1促进剂活性的功能作用.
主要成果:
- 在PBMC中FOXO1转录表达与AR风险 (p < 0.05) 和TNSS (p < 0.0001) 显著相关.
- 标签-SNP rs9549246显示了与FOXO1转录水平和AR风险 (OR = 1.21,p = 0.044) 的显著关联.
- rs35594717的小等位基因"A" (标记为rs9549246) 在体外增强了FOXO1促进体活性.
结论:
- 在PBMC中FOXO1转录水平与AR风险和症状严重程度密切相关.
- 基因变异,特别是那些被rs9549246标记的变异,会影响FOXO1的表达,并导致新加坡/马来西亚人群的AR发展.
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