在骨肌肉细胞外矩阵中的原纤维的应变依赖的动态重新调整
Ross P Wohlgemuth1, Sathvik Sriram1, Kyle E Henricson1
1Department of Neurobiology, Physiology, & Behavior, University of California Davis, United States.
Acta biomaterialia
|August 29, 2024
概括
骨肌肉的原纤维动态地重新定向并与伸展保持一致,影响被动肌肉性. 这项研究可视化和量化了对肌肉疲劳的反应中的原对齐变化,揭示了细胞外矩阵力学的洞察力.
科学领域:
- 生物物理学的生物物理.
- 骨肌肉生理学 骨肌肉生理学
- 细胞外矩阵生物学 细胞外矩阵生物学
背景情况:
- 骨肌肉细胞外基质 (ECM) 中的原纤维结构对于被动肌肉力学至关重要.
- 在肌肉拉伸过程中缺乏动态可视化和对原纤维重定向的量化.
研究的目的:
- 测量不同肌肉长度的原对齐变化,并将它们与被动肌肉力学相关联.
- 为了测试这种假设,原纤维动态增加与伸展对齐,影响肌肉硬.
- 研究原酶消化对原蛋白重新调整的影响及其与肌肉机制的关系.
主要方法:
- 来自小鼠的隔离扩展体长肌,肌和腹膜肌肉.
- 用原酶或假溶液处理肌肉,然后进行脱细胞化,以创建脱细胞化肌肉结构 (DCM).
- 经过机械测试的DCM并使用第二子代显微镜来量化不同菌株的原蛋白对齐.
主要成果:
- 原蛋白对齐增加了以一种取决于菌株的方式.
- 原酶治疗没有显著改变对抗菌株依赖的原对齐变化.
- 隔膜表膜和周围膜的原纤维,尽管从不同的角度开始,但在拉伸时以同样的方式重新定位.
- 观察到的原对齐的变化显示,与被动DCM刚度的弱相关性.
结论:
- 骨肌肉细胞外矩阵原蛋白结构是动态的,沿着拉伸轴重新定向.
- 增加原蛋白对齐与被动肌肉硬度的关系很弱.
- 肌肉细胞外矩阵的刚性取决于不仅仅是原交叉连接和对齐的因素,这表明成分的复杂相互作用.
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