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Updated: May 5, 2026

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生物工程基于脂质的合成细胞用于治疗性蛋白质输送

Sónia Siquenique1, Shanny Ackerman2, Avi Schroeder2

  • 1i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal.

Trends in biotechnology
|August 29, 2024
PubMed
概括

合成细胞 (SCs) 通过模仿自然细胞提供先进的治疗性蛋白质输送. 这些基于脂质的系统可实现针对性和受控的蛋白质生产,用于增强药物输送应用.

关键词:
没有细胞的基因表达.没有细胞的蛋白质合成.合成细胞的合成细胞.治疗蛋白质的治疗蛋白质.

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Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
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