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DNA Bacteriophages

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...

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Updated: May 11, 2026

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

通过菌体显示与高通量测序相结合的细菌细胞表面的表征.

Casey N Grun¹, Ruchi Jain^2,3, Maren Schniederberend²

¹Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT, USA.

Nature communications

|August 29, 2024

概括

一种新的方法,Phage-seq,使用菌体显示和DNA测序来快速识别细菌细胞表面的变化. 这项技术有助于了解抗菌素耐药性,并开发用于Pseudomonas aeruginosa感染的新诊断和治疗方法.

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Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

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Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

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Last Updated: May 11, 2026

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

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Published on: January 17, 2015

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

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Published on: October 3, 2018

科学领域:

微生物学微生物学
免疫学免疫学免疫学
基因组学就是基因组学.

背景情况:

抗微生物耐药性是由细菌适应选择性压力的驱动.
Pseudomonas aeruginosa 长期感染并进化以抵抗抗微生物药物并逃避宿主防御.
鉴定细菌细胞表面的适应性变化是具有挑战性的,因为方法有限.

研究的目的:

开发一种高通量,多重复合技术,用于测量细菌细胞表面.
为了识别Pseudomonas aeruginosa细胞表面的适应性变化.
为了产生针对细菌毒性因子的新型抗体.

主要方法:

将菌体显示与高通量DNA测序相结合,创建菌体序列.
在数百种细菌基因型中应用菌体显示分析.
分析菌体显示选择动态,以捕获细胞表面信息.

主要成果:

基测量使细菌细胞表面的高通量,多重测量成为可能.
该方法确定了Pseudomonas aeruginosa细胞表面的关键适应性变化.
产生了识别活细胞上的P. aeruginosa毒性因子的Camelid单域抗体.

结论:

基测量是研究细菌细胞表面的强大范式.
这项技术可促进许多表面特征的并行识别和分析.
产生的抗体在细菌感染的诊断和治疗中具有潜在的应用.