控制YY1的调节连接和转录受细胞周期的影响
Jessica C Lam1,2, Nicholas G Aboreden1,2, Susannah C Midla1
1Division of Hematology, The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Nature genetics
|August 29, 2024
概括
阴阳1 (YY1) 在不影响染色体区间的情况下维持红状腺细胞中的增强剂-促进剂循环. 它的细胞周期依赖的结合影响在G1阶段进入期间循环的建立.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 细胞生物学 细胞生物学
背景情况:
- 转录因子通过调解增强剂和促进剂之间的相互作用,在基因调节中发挥关键作用.
- 转录因子的结构功能,包括它们与凝聚素和CTCF的相互作用,对于组织色素结构至关重要.
- 了解转录因子在细胞周期中的动态作用,对于理解基因调节至关重要.
研究的目的:
- 调查阴阳1 (YY1) 在红状腺细胞中建立和维持增强剂-促进剂循环中的作用.
- 为了确定YY1在染色质结构中的功能是否依赖于凝聚素介导的循环挤出.
- 阐明细胞循环进展,特别是线粒分裂到G1转变对YY1在增强剂-促进剂相互作用中的作用的影响.
主要方法:
- 在红状腺细胞中YY1的急性降解.
- 增强器-促进器循环,区块和域的分析.
- 调查凝聚和循环挤出的参与.
- 在细胞循环期间跟踪YY1动态 (线粒分裂到G1).
主要成果:
- YY1是维护众多增强器-促进器循环所需的,但不是分区或域.
- 依赖于YY1的循环形成不依赖于停滞的凝聚力介导的循环挤出.
- YY1在促进体的线粒体染色体上被部分保留,并在线粒体退出时迅速重新结合,与循环建立相吻合.
- 循环的子集需要YY1在G1进入期间建立,但不是用于相间维护,突出显示细胞循环依赖的功能.
结论:
- YY1 作为一种关键的结构蛋白,在红状腺细胞中维护增强剂-促进剂循环.
- YY1的染色体结构功能与凝聚素介导的循环挤出不同,受细胞周期阶段的影响.
- 转录因子,凝聚素,CTCF和细胞循环动态之间的相互作用塑造了高阶染色体组织.
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