使用乳酸细菌 (LAB) 消除脂聚糖 (LPS)
1Department of Food Management, Miyagi University, Sendai, Japan. kanauchi@myu.ac.jp.
Methods in molecular biology (Clifton, N.J.)
|August 29, 2024
概括
乳酸细菌 (LAB) 可以消除脂多糖 (LPS),一种导致肠道炎症的化合物. 一些LAB菌株,特别是Pediococcus pentosaceus,有效地去除LPS,为益生菌应用提供了潜力.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 像大肠杆菌 (Escherichia coli) 这样的阴性细菌产生脂多糖 (LPS),一种已知的强效内毒素,可诱导肠道炎症.
- 乳酸细菌 (LAB) 以它们对肠道健康的有益影响而闻名,包括预防细菌性腹.
研究的目的:
- 用乳酸细菌 (LAB) 描述一种消除LPS的方法.
- 为了识别具有LPS消除活性的LAB菌株,并描述有效的细胞分数.
主要方法:
- 脂聚糖化物 (LPS) 度的测定是使用LPS测试套件进行的,该套件采用了临层级反应.
- 几种LAB菌株,包括四种细菌和一种菌株 (Pediococcus pentosaceus),被测试了LPS消除活性.
- 研究室的细胞分量 (细胞外,细胞膜,细胞质,细胞壁) 被分离出来,并对其消除LPS的能力进行了评估.
主要成果:
- 四种细菌菌株和一种菌株 (Pediococcus pentosaceus) 显示出LPS消除活性.
- 皮迪奥科克斯 (Pediococcus pentosaceus) 清除了高达43%的LPS.
- 细胞壁消化分数显示了最高的LPS消除活性,减少了45%的LPS.
- 在特定条件下,LAB有效地用O-抗原消除了LPS:在15-30°C的低糖介质中.
结论:
- 乳酸细菌,特别是Pediococcus pentosaceus,具有显著的LPS消除活性.
- LAB的细胞壁部分主要负责LPS的消除.
- 这种方法提供了一个精确而简单的方法来评估LAB在益生菌的背景下消除LPS.
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