从牛奶油中分离出来的Enterococcus faecium PCH.25中的抗菌素和抗氧化剂外聚糖:特征和益生菌潜力
Parvin Chegini1, Fatemeh Salimi2,3, Zahra Abdollahpour Pirbodagh4
1Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, 36716-41167, Iran.
Archives of microbiology
|August 29, 2024
概括
一种来自黄油的新型Enterococcus faecium菌株产生具有益生菌潜力的外聚糖. 外聚糖表现出抗菌和抗氧化物质的特性,表明在食品和制药中的应用.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
背景情况:
- 乳酸细菌外聚糖被认为具有健康益处和功能性食品应用.
- 新型产出外聚糖的菌株需要隔离和鉴定其潜在的工业用途.
研究的目的:
- 从乳制品中分离和表征一种新型的外聚糖生成菌株.
- 评估孤立菌株及其外聚糖的益生菌潜力.
- 为了研究产生的外聚糖的生物活性和物理化学特性.
主要方法:
- 从牛油中分离和识别出产外聚糖的细菌.
- 益生菌潜力的评估:耐酸性,自聚,协聚,抗生素敏感性,血液溶解活性.
- 外聚糖的特征:对Listeria monocytogenes的抗菌活性,抗氧化性质,化学成分,热稳定性,泽塔潜力,颗粒大小,SEM,EDX分析.
主要成果:
- 分离了Enterococcus faecium PCH.25,并证明了外聚糖的产生.
- 该菌株表现出高酸性耐受性 (92.24%在pH2下可活性),显著的自身聚合 (85.27%) 和抗生素敏感性,表明益生菌潜力.
- 外聚糖具有针对Listeria monocytogenes的杀菌活性 (MIC/MBC = 1.8 mg/ml),抗氧化特性 (22.8%),高达270°C的热稳定性以及光滑无形结构.
结论:
- 菌 (Enterococcus faecium PCH.25) 是一个有前途的益生菌候选人.
- 由E. faeciumPCH.25产生的外聚糖具有显著的抗菌和抗氧化特性.
- 这种外聚糖在食品,化品和制药行业作为天然聚合物具有潜在的应用.
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