相关实验视频

Updated: Jun 14, 2025

Isolation of Tissue Extracellular Vesicles from the Liver

Isolation of Tissue Extracellular Vesicles from the Liver

Published on: August 21, 2019

肝细胞衍生的组织细胞外囊泡保护肝脏的再生,并支持再生疗法

Si-Qi Ying¹, Yuan Cao^1,2, Ze-Kai Zhou^1,3

¹State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi International Joint Research Center for Oral Disease, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China.

Journal of nanobiotechnology

|August 29, 2024

概括

肝脏组织的细胞外囊泡 (EVs),特别是肝细胞衍生的EVs (Hep-EVs),在受伤后驱动肝脏的再生. 补充这些EV显示肝脏修复的治疗潜力.

科学领域:

细胞生物学细胞生物学
再生医学是一种再生医学.
细胞外囊泡细胞外囊泡

背景情况:

组织衍生的细胞外囊泡 (EVs) 对于器官平衡至关重要,但它们在再生中的作用还未得到充分研究.
了解肝组织EVs的功能和治疗潜力对于推进再生医学至关重要.

研究的目的:

研究在部分肝切除术 (PHx) 后肝脏再生中 in situ 肝组织 EVs (LT-EVs) 的作用.
确定参与肝脏修复的EVs的来源和功能机制.
探索肝细胞衍生的EVs (Hep-EVs) 在改善肝脏再生不足方面的治疗潜力.

主要方法:

大量和单细胞RNA测序分析.
超结构组织检查.
在纳米尺度和EVs的蛋白质分子分析.
在体内抑制EV释放使用AAV-shRab27a.a.

主要成果:

肝细胞是再生肝脏中EVs的主要来源.
肝细胞特异性EVs (Hep-EVs) 携带增殖信号,对于编排肝脏再生至关重要.
乙肝-EVs通过循环依赖激酶1 (Cdk1) 活性促进细胞循环进展,促进肝细胞的增殖.
补充再生性Hep-EVs改善了肝脏再生不足.

结论:

再生性Hep-EVs的释放是快速肝脏再生的关键调节者.
这项研究建立了内源性EVs在器官再生中的功能和机制框架.
再生性Hep-EVs在肝疗法中具有显著的翻译潜力.

关键词:

细胞循环的细胞周期细胞外囊泡中的细胞外囊泡.肝细胞肝细胞肝细胞肝脏的再生再生部分肝切除术部分肝切除术

更多相关视频

An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue

An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue

Published on: February 3, 2023

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy

Published on: June 29, 2021

相关概念视频

Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...

Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...

Stem Cell Therapy for Tissue Regeneration

Stem Cell Therapy for Tissue Regeneration

Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...