一个精子丰富的5'片段的tRNA-Valine调节了植入前胚胎转录组和发育
Simeiyun Liu1, Andrew D Holmes1, Sol Katzman2
1Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, California, 95064.
bioRxiv : the preprint server for biology
|August 30, 2024
概括
精子小RNA,如tRFValCAC,通过细胞外囊泡传递给精子,并通过控制mRNA剪接来调节早期胚胎发育. 这揭示了父亲表观遗传的一种新机制.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
- 发展生物学 发展生物学
背景情况:
- 精子小RNA是父亲表观遗传的关键,但它们的起源和作用尚不清楚.
- tRFValCAC,一个tRNA片段,在精子中很丰富,并且在表皮质成熟过程中得到丰富.
- 流行性细胞通过细胞外囊泡将tRFValCAC传递给精子.
研究的目的:
- 研究tRFValCAC向精子输送的机制.
- 确定tRFValCAC在胚胎早期发育中的功能.
- 阐明精子小RNA在代际表观遗传中的作用.
主要方法:
- 确定tRFValCAC是一种精子丰富的小RNA.
- 研究了tRFValCAC与hNRNPAB的相互作用,用于将其分类为细胞外囊泡.
- 在植入前胚胎中抑制tRFValCAC以评估其功能.
- 分析了对mRNA处理,拼接和胚胎发育的影响.
主要成果:
- tRFValCAC 与 hnRNPAB 相互作用,调节其包装成用于精子输送的细胞外囊泡.
- 在胚胎中抑制tRFValCAC改变了mRNA处理和拼接基因表达.
- tRFValCAC抑制的胚胎表现出异常的mRNA拼接和延迟的植入前发育.
- tRFValCAC在调节替代拼接和胚胎发育方面发挥着至关重要的作用.
结论:
- 由精子衍生的tRFValCAC,通过表皮细胞外囊泡传递,调节胚胎mRNA剪接和发育.
- 这项研究揭示了精子小RNA功能在早期胚胎发生过程中的新机制.
- 这些发现提供了关于精子小RNA介导的父环境影响的表观遗传的见解.
更多相关视频
09:22Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
Published on: February 9, 2015
22.6K
08:00Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
Published on: June 16, 2021
4.3K
相关概念视频
01:23Regulation of Expression at Multiple Steps
875
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
875
02:01pre-mRNA Processing
52.8K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
52.8K
01:35Pre-mRNA Processing: Modification of pre-mRNA Ends
9.2K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
9.2K
02:36Transfer RNA Synthesis
11.9K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
11.9K
02:24Regulation of Expression Occurs at Multiple Steps
22.5K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.5K