一种感染MDR Salmonella enterica的新型菌体及其应用作为有效的食品生物控制
Anu Bala Jaglan1,2, Ravikant Verma2, Medhavi Vashisth1
1ICAR - National Research Centre on Equines, Hisar, India.
Frontiers in microbiology
|August 30, 2024
概括
一种新型的菌体phiSalP219有效地向多药耐药的沙门氏菌,为食品安全提供了抗生素的有希望的替代品. 这种菌体表现出广泛的活性和生物膜根除能力.
科学领域:
- 微生物学 微生物学
- 病毒学 病毒学
- 食品安全 食品安全
背景情况:
- *Salmonella enterica*的抗生素耐药性构成了严重的公共卫生威胁.
- 菌体是对抗耐药细菌的可行的替代策略,因为它们的特异性和抗菌膜活性.
研究的目的:
- 为了隔离和描述一种新型细菌对抗多抗药性*Salmonella enterica*有效的新型细菌.
- 为了评估菌体在控制*沙门氏菌*感染和食品矩阵中的生物膜方面的有效性.
主要方法:
- 从池水中分离出一种具有广泛宿主范围的菌体 (phiSalP219),对抗多药耐药的*Salmonella enterica*血清型.
- 通过电子显微镜和基因组分析对菌体形态的表征.
- 对各种*沙门氏菌*菌株的菌体活动的评估,在酸性环境和高温下.
- 对菌体安全性和有效性进行评估,以控制准备食用的肉中的*Salmonella*Typhimurium和消除生物膜.
主要成果:
- 菌 phiSalP219 在30种测试的 *Salmonella enterica* 菌株中解析了28种.
- 菌体在酸性条件 (pH3) 和高温 (70°C) 中表现出稳定性.
- 基因组分析证实phiSalP219是一种属于*Caudoviricetes*类的菌体,具有146Kb的基因组和250个预测的CDS.
- 发现菌素phiSalP219在微生物学上是安全的,并且有效控制肉类中的*S. enterica*血清 Typhimurium,并消除其生物膜.
结论:
- 菌体phiSalP219是一种强效的,对抗多药耐药性*Salmonella enterica*的解毒剂.
- 它在恶劣条件下的稳定性和抗菌膜活性使其成为食品安全应用的有希望的候选者.
- PhiSalP219代表了一种安全有效的替代方案,用于对抗抗生素耐药的 * 沙门氏菌 * 在食品中的感染.
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