一种修改后的消毒和储存方法,用于结核病患者的唾液
Nguyen Le Quang1, Do Dang Anh Thu1, Le Pham Tien Trieu1
1Oxford University Clinical Research Unit, Centre for Tropical Medicine, Ho Chi Minh city, Vietnam.
Wellcome open research
|August 30, 2024
概括
一种经过修改的唾液处理方法提高了储存能力和Mycobacterium tuberculosis的回收,以便在未来培养. 这种方法最大限度地减少了实验室的工作量和污染风险,对于大规模的样本采集证明是有效的.
科学领域:
- 微生物学 微生物学
- 临床诊断 临床诊断 临床诊断
- 公共卫生 公共卫生
背景情况:
- 对样品的有效处理对于未来的结核菌菌 (M.tb) 培养至关重要.
- 目前的方法需要优化以最大化存储和M.tb恢复.
- 尽量减少实验室工作量和污染风险对于大规模样本处理至关重要.
研究的目的:
- 开发和评估一种修改后的唾液消化和消毒方法.
- 评估修改方法对存储能力,M.tb回收和实验室效率的影响.
- 为了将修改后的方法与标准的N-乙-L-氨酸和氧化 (NALC-NaOH) 方法进行比较.
主要方法:
- 来自肺结核患者的唾液样本使用标准NALC-NaOH和修改方法进行处理.
- 经过处理的样本被冷,然后在BD BACTEC 960菌根生长指标管 (MGIT) 中进行培养.
- 分析了时间到阳性 (TPP) 和增长单位 (GU) 数据,以及污染率.
主要成果:
- 与标准方法相比,修改后的方法产生了较长的TPP (p < 0.05),但与GU相似.
- 在最初的比较测试中,观察到修改方法的低失败率 (1/22).
- 在一个更大的队列 (348个样本) 中,修改后的方法显示MGIT阳性率为90.5%,阴性率为7.8%,污染率为1.7%,存储时间或涂抹质量没有显著影响.
结论:
- 修改后的唾液处理方法在长时间内收集和存储大量样品时是有效的.
- 它实现了可接受的M.tb恢复率,并保持了低污染风险.
- 这种方法为未来的诊断和研究目的的大规模M.tb样本管理提供了可行的解决方案.
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