在医院环境中的消毒剂中细菌分离物的生存机制的深入研究
Pue Rakshit1, Aradhana Singh1, Ravindra Singh2
1Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Frontiers in cellular and infection microbiology
|August 30, 2024
概括
医院中的细菌正在适应生存消毒剂,导致多药性耐药性和生物膜形成. 这种适应对医疗保健机构的感染控制构成了重大挑战.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 医院流行病学 医院流行病学
背景情况:
- 消毒剂耐药性是一个日益增长的威胁,减少了必要的感染控制措施的有效性.
- 医院环境,特别是交通繁忙的地区,可以容纳对消毒剂产生耐药性的细菌.
研究的目的:
- 研究细菌适应策略,以应对消毒剂暴露.
- 确定在医院获得的病原体中对消毒剂耐药性的机制.
主要方法:
- 从医院洗手中分离并确定了细菌菌株 (Klebsiella pneumoniae,Pseudomonas aeruginosa).
- 确定六种常见消毒剂的最小抑制度 (MIC) 和最小杀菌度 (MBC).
- 分析了抗生素耐药性,生物膜形成,并使用全基因组测序 (WGS) 来识别耐药性基因.
主要成果:
- 在暴露于甲 (BAC) 后,隔离体表现出抗性增加 (MIC/MBC翻倍).
- 大多数分离物是多药耐药的,形成强大的生物膜,在更高的消毒剂度下降了生物膜抑制.
- 工作组确定了抗菌素耐药性 (AMR) 基因,包括blaDIM-1,消毒剂耐药性基因和排泄基因.
结论:
- 细菌表现出重要的适应策略,在富含消毒剂的医院环境中生存.
- 这些适应,包括多药性耐药性和增强的生物膜形成,在医疗机构控制感染方面构成了重大挑战.
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