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从大脑子区域中快速和稳健地分离微质细胞和血管细胞,用于整合性单细胞分析

Efthalia Preka1, Alejandro Lastra Romero1, Ying Sun2

  • 1Department of Women's and Children's Health, Biomedicum A4, Karolinska Institutet, 171 77, Stockholm, Sweden.

Heliyon
|August 30, 2024
PubMed
概括

目前的大脑细胞隔离方法对于转录学研究来说太慢了. 这种新的快速协议有效地将小质细胞和血管细胞从小脑样本中分离出来,用于分子分析.

关键词:
内皮细胞是内皮细胞.神经炎症是一种神经炎症.细胞 (Pericytes) 是一种细胞.有关RNA测序的RNA测序血管疾病是血管疾病.

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.