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甲基二硫氧化物减弱了电离辐射诱导的人类诱导多能干干细胞中中核细胞重复和多极细胞分裂的作用

Mikio Shimada¹, Ryoichi Hirayama², Yoshihisa Matsumoto¹

¹Laboratory for Zero-Carbon Energy, Institute of Innovative Research, Tokyo Institute of Technology, Tokyo, 152-8550, Japan.

Radiation research

|August 30, 2024

概括

电离辐射导致人体诱导多能干细胞 (hiPSCs) 中的中心细胞过重复. 与二甲基硫氧化物 (DMSO) 结合辐射抑制了这种效应,为再生医学提供了关于干细胞基因组稳定性的见解.

科学领域:

细胞生物学细胞生物学
遗传学是一个遗传学.
再生医学是一种再生医学.

背景情况:

中心体对于细胞分裂和基因组稳定性至关重要.
电离辐射可以破坏细胞循环调节,导致中枢细胞过重复和潜在的瘤产生.
多能干细胞 (PSC) 具有独特的细胞周期动态,这可能会影响它们对DNA损伤的反应.

研究的目的:

研究外源性DNA损伤对人类诱导多能干细胞 (hiPSCs) 中细胞周期依赖的中心体调节的影响.
为了比较hiPSCs与分化细胞的辐射反应.
探索减轻hiPSCs中辐射诱导的中心体异常的方法.

主要方法:

利用从皮肤纤维细胞中获得的人类诱导多能干细胞 (hiPSCs) 作为模型系统.
将hiPSC暴露在电离辐射中.
在与辐射相结合的情况下,给予激素清除剂二甲基硫氧化物 (DMSO).
评估中心细胞数,DNA损伤和细胞分裂模式.

主要成果:

与差异化细胞相比,hiPSCs在暴露于辐射后表现出更高的中心细胞过重复和多极细胞分裂频率.
用辐射和DMSO进行的联合治疗显著减少了hiPSC中的DNA损伤和中心体过重复.
DMSO减轻了辐射对hiPSCs的间接影响.

结论:

hiPSCs比分化的细胞更容易受到辐射诱导的中心体过重复.
甲基二硫化物 (DMSO) 可以保护hiPSCs免受辐射诱导的基因组不稳定性.
了解干细胞对DNA损伤的反应对于评估再生医学中的风险至关重要.

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