基于纳米体的重链抗体和嵌合抗体
1Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Immunological reviews
|August 30, 2024
概括
驼类动物进化出独特的纳米体,它们是单个抗体域. 这些纳米体可以被设计成新型重链抗体,用于治疗应用.
科学领域:
- 免疫学 免疫学 免疫学
- 进化生物学 进化生物学
- 生物技术是生物技术.
背景情况:
- 像驼和驼这样的驼类动物,除了常规抗体外,还具有独特的免疫球蛋白系统.
- 这个系统包括由缺乏CH1域的重链组成的同质重链抗体,称为纳米体.
- 纳米体是一个进化发明,可以追溯到大约4500万年前的驼类的共同祖先.
研究的目的:
- 探索纳米体和纳米体衍生的重链抗体的性质和潜在应用.
- 突出这些独特结构作为免疫疗法的潜力.
主要方法:
- 从免疫动物或合成图书馆获得对人类抗原的纳米体的衍生.
- 通过将它们与其他蛋白质融合,包括其他纳米体或免疫球蛋白域,来设计纳米体.
主要成果:
- 纳米体是坚固的,高度溶解的,单个免疫球蛋白域.
- 它们可以很容易地与其他蛋白质融合,从而创建新的抗体格式.
- 来自纳米体的重链抗体显示出作为新一类免疫疗法药物的潜力.
结论:
- 纳米体是一种独特的抗体类别,具有独特的结构和功能性质.
- 它们在蛋白质工程中的多功能性为开发先进的免疫疗法开辟了道路.
更多相关视频
13:14Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
Published on: February 6, 2018
10.4K
08:09Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy
Published on: April 6, 2015
11.6K
相关概念视频
01:25Antibody Structure and Classes
856
Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
856
01:10Antibody Structure
59.6K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
59.6K
01:31Hybridoma Technology
14.2K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
14.2K