在突尼斯的废水中追踪SARS-CoV-2及其变体
Ines Othman1, Maxime Bisseux2, Amna Helmi3
1Faculty of Pharmacy, University of Monastir, LR99-ES27, Monastir, Tunisia
Journal of water and health
|August 30, 2024
概括
在突尼斯的废水基因组监测检测到SARS-CoV-2变种,包括在官方承认之前的BA.5. 废水中的病毒RNA预测了COVID-19病例的增加,突出了净化厂的低效率.
科学领域:
- 环境微生物学环境微生物学
- 基因组流行病学基因组流行病学
- 公共卫生监督是对公共卫生的监督.
背景情况:
- 基于废水的流行病学 (WBE) 为社区病原体流行率提供了有价值的见解.
- 废水的基因组监测有助于追踪新兴变种和了解传播动态.
研究的目的:
- 在突尼斯实施WBE用于SARS-CoV-2监测.
- 在废水中识别和跟踪SARS-CoV-2变种.
- 评估废水处理厂 (WWTP) 在消除SARS-CoV-2方面的有效性.
主要方法:
- 分析了来自突尼斯六个废水处理厂的废水流入和排放情况 (2021年12月至2022年7月).
- 使用逆转录固体数字PCR (RT-sdPCR) 来定量SARS-CoV-2RNA.
- 通过全基因组测序识别SARS-CoV-2血统.
主要成果:
- 废水中的病毒RNA度与报告的COVID-19病例相关,往往在临床诊断之前增加.
- 包括BA.5在内的Delta和Omicron变种在突尼斯的废水中被检测到.
- 在经过处理的废水中存在SARS-CoV-2RNA,这表明WWTPs的清除不完全.
结论:
- 废水基因组监测有效监测突尼斯的SARS-CoV-2流行率和变种的出现.
- 在废水中早期发现像BA.5这样的变种提供了重要的公共卫生信息.
- 突尼斯目前的废水处理方法需要改进,以有效地去除SARS-CoV-2.
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