照相交联原酸作为生物墨水在基于挤出的3D生物打印中的表征和应用
Po-Hsun Chen1, I-Hsiang Chen1, Wei-Hsiang Kao1
1Department of Chemical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 106, Taiwan. weibortsai@ntu.edu.tw.
Biomaterials science
|August 30, 2024
概括
研究人员开发了一种新的原生物墨水 (ColME) 用于3D生物打印,提高了溶解性和可打印性. 这种新型材料支持强大的,细胞活性的组织制造,具有改进的机械性能.
科学领域:
- 生物制造的生物制造
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
背景情况:
- 3D生物打印提供了精确的组织结构制造.
- 原蛋白是一种标准的生物材料,由于其特性,对生物墨水提出了制定挑战.
- 开发改进的生物墨水对于推进3D生物打印应用至关重要.
研究的目的:
- 为3D生物打印引入一种新型,中性溶解,可光交联的原衍生物 (ColME).
- 为了优化ColME生物墨水特性和生物打印参数.
- 评估基于ColME的3D构造的可打印性,结构完整性和细胞活力.
主要方法:
- 合成的原酸 (ColME) 通过修改牛类型I原与酸.
- 优化了3D生物打印参数,包括生物墨水度,挤压压力,喷嘴速度和温度.
- 使用显微镜,机械测试和细胞活力测试评估构造质量.
主要成果:
- 在生理pH值下,ColME具有更高的溶解度,同时保持了原的三螺旋结构.
- 优化印刷条件 (较低的温度,较小的喷嘴) 改善了网格结构质量.
- 间歇性光交联使得能够制造出高细胞活性的强大,多层的3D结构.
- 与甲基化凝相比,ColME表现出更高的机械强度和酶抗性.
结论:
- 原酸 (ColME) 是一个有前途的,多功能生物墨水,用于3D生物打印.
- 科尔米 (ColME) 便于创建结构健全且与细胞兼容的3D组织结构.
- 这一进步有潜力开发耐用,生物活性的工程组织.
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