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温度和湿度对昆虫DNA完整性的影响,通过实时PCR评估

Elizabeth V Fowler1,2, Melissa L Starkie1, Mark J Blacket3

  • 1Department of Agriculture and Fisheries, Biosecurity Queensland, Brisbane, QLD, Australia.

Journal of economic entomology
|August 30, 2024
PubMed
概括

高温和高湿度加速了昆虫DNA的降解,影响了害虫监测. 较短的陷清除时间和改进的陷设计是准确的生物安全诊断的建议.

关键词:
维护DNA的保护它们是Tefritidae的动物.生物安全 生物安全在gBlock上,您可以使用gBlock.

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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